SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25663

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1301 expressed in HEK293

Format

Azide and BSA Free
View all SARS-CoV-2 nsp7 Primary Antibodies »

Product Specifications

Immunogen

Full length SARS-CoV-2 nsp7 recombinant protein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

Immunohistochemistry-Paraffin: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] -

Immunohistochemistry-Paraffin: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] - SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] detects SARS-CoV-2 (COVID-19) nsp7 protein at cytoplasm by immunohistochemical analysis. Sample: SARS-CoV-2 (COVID-19) nsp7 transfected 293T cell FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) nsp7 stained by SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (NBP3-25663) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] -

Immunocytochemistry/Immunofluorescence: SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free [NBP3-25663] - SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] detects SARS-CoV-2 (COVID-19) nsp7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp7 stained by SARS-CoV-2 (COVID-19) nsp7 antibody [HL1301] (NBP3-25663) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10um.
SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

Western Blot: SARS-CoV-2 nsp7 Antibody (HL1301) [NBP3-25663] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 nsp7 antibody [HL1301] (NBP3-25663) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp7

SARS-CoV-2 Nonstructural Protein 7 (NSP7) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP7 is 83 amino acids (aa) with a theoretical molecular weight of 9.2 kDa (1,2). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP7 has 98.8% sequence identity and 100% sequence similarity (1). Functionally, SARS-Cov-2 NSP7 forms a complex with NSP8 and NSP12 to generate NSP8's RNA polymerase activity (1-3). In SARS-CoV, NSP7 and NSP8 are required for viral survival and form a channel-like, cylinder supercomplex (3). The NSP7-NSP8-NSP12 complex is considered the minimal core complex for RNA synthesis (4). While structurally similar to SARS-CoV, the components of the NSP7-NSP8-NSP12 complex of SARS-CoV-2 have reduced polymerase activity and thermostability (4).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M.,... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Peng, Q., Peng, R., Yuan, B., Zhao, J., Wang, M., Wang, X., Wang, Q., Sun, Y., Fan, Z., Qi, J., Gao, G. F., & Shi, Y. (2020). Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2. Cell reports. https://doi.org/10.1016/j.celrep.2020.107774

Alternate Names

Non-structural protein 7, nsp7 replicase

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp7 Products

Product Documents for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 nsp7 Antibody (HL1301) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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