SARS-CoV-2 Nucleocapsid Antibody (004) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-12734

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

SARS-CoV

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 004

Format

Azide and BSA Free
View all SARS-CoV-2 Nucleocapsid Primary Antibodies »

Product Specifications

Immunogen

Recombinant SARS-CoV-2 Nucleocapsid Protein

Specificity

Has cross-reactivity in ELISA and WB with:SARS-CoV-2 Nucleocapsid ProteinHas cross-reactivity in ELISA with:SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid ProteinSARS-CoV-2 Nucleocapsid ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) ProteinSARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) ProteinSARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) ProteinSARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) ProteinSARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) ProteinSARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) ProteinSARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) ProteinSARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) ProteinSARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) ProteinNo cross-reactivity in ELISA with:MERS-CoV Nucleocapsid proteinHCoV-229E Nucleocapsid proteinHCoV-NL63 Nucleocapsid proteinHCoV-HKU1(isolate N5) Nucleocapsid proteinHCoV-OC43 Nucleocapsid protein

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SARS-CoV-2 Nucleocapsid Antibody (004) - Azide and BSA Free

Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Lane A: SARS-COV-2 Nucleocapsid overexpressed HEK293 Whole Cell LysateLane B: HEK293 Whole Cell LysateLysates/proteins at 10 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing condition
Flow Cytometry: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Flow Cytometry: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Flow Cytometry: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Analysis of SARS-COV-2 Nucleocapsid overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Nucleocapsid rabbit MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Western Blot: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734] - Anti-SARS-NP rabbit monoclonal antibody at 1:1000 dilution.Lane A: SARS-CoV NP Protein Lane B: SARS-CoV NP Protein Lane C: SARS-CoV-2 (2019-nCoV) NP Protein Lane D: SARS-CoV-2 (2019-nCoV) NP Protein SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.
SARS-CoV-2 Nucleocapsid Antibody (004) Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Immunohistochemistry: SARS-CoV-2 Nucleocapsid Antibody (004) [NBP3-12734]

Immunochemical analysis of SARS-CoV-2 Nucleocapsid overexpressed Hek293 Cells stained with NBP3-12734, then a HRP-conjugated secondary antibody.

Applications for SARS-CoV-2 Nucleocapsid Antibody (004) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:1000-1:10000
Application Notes
Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 Nucleocapsid

The SARS-CoV-2 Nucleocapsid protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The nucleocapsid protein is an RNA-binding protein that is essential for viral assembly into a ribonucleoprotein complex and also functions in viral budding (2,3). More specifically, after viral replication and RNA synthesis, the viral genomes that are encapsulated by the nucleocapsid protein will bud into the membrane and help with mature virus formation (2). The nucleocapsid protein is synthesized as 419 amino acids (aa) in length with a theoretical molecular weight of 45.6 kDa (4). Structurally, the nucleocapsid protein contains an RNA-binding domain (N1b), a dimerization domain (N2b), and a few shorter regions (N1a, N2a, and spacer B/N3) (3). It is the N1b domain that is suggested to be involved in RNA binding (3). The nucleocapsid N1b domain contains a beta-hairpin, a loop region, and a beta-sheet core, giving it a right-handed shape (3). Conversely, the N2b domain functions in dimerization which promotes assembly into a helical filament where the dimer interface is made of two beta-strands and an alpha-helix portion (3). Inhibition of nucleocapsid assembly may be a potential target for treating COVID-19 infection.

Following SARS-CoV-2 infection, B cells and T cells display an immune response against nucleocapsid protein and nucleocapsid-specific neutralizing antibodies are produced (5). Interestingly, a study of patients who have recovered from COVID-19 revealed that there were no neutralizing antibodies against nucleocapsid protein present; however, a high presence against neutralizing spike RBD antibodies were detected (5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Zhu, G., Zhu, C., Zhu, Y., & Sun, F. (2020). Minireview of progress in the structural study of SARS-CoV-2 proteins. Current Research in Microbial Sciences. https://doi.org/10.1016/j.crmicr.2020.06.003

4. Uniprot (P0DTC9)

5. Shah, V. K., Firmal, P., Alam, A., Ganguly, D., & Chattopadhyay, S. (2020). Overview of Immune Response During SARS-CoV-2 Infection: Lessons From the Past. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01949

Alternate Names

2019-nCoV N Protein, 2019-nCoV Nucleocapsid Protein, COVID-19 N protein, COVID-19 nucleocapsid protein, Human coronavirus Nucleoprotein, nucleocapsid phosphoprotein, Nucleocapsid protein, Nucleoprotein, ORF9; structural protein, SARS-CoV-2, SARS-CoV-2 Nucleocapsid protein, SARS-CoV-2 N protein, SARSCoV2 Nucleoprotein, Severe Acute Respiratory Syndrome Coronavirus 2 Nucleocapsid Protein

Gene Symbol

N

Additional SARS-CoV-2 Nucleocapsid Products

Product Documents for SARS-CoV-2 Nucleocapsid Antibody (004) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 Nucleocapsid Antibody (004) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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