SATB1 Antibody (OTI13D6)

Novus Biologicals | Catalog # NBP1-47950

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI13D6
View all SATB1 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SATB1 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens SATB homeobox 1 (SATB1), transcript variant 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SATB1 Antibody (OTI13D6)

Western Blot: SATB1 Antibody (13D6) [NBP1-47950] Analysis of extracts (35ug) from 9 different cell lines by using anti-SATB1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunocytochemistry/ Immunofluorescence: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunocytochemistry/Immunofluorescence: SATB1 Antibody (13D6) [NBP1-47950] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SATB1.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SATB1 mouse monoclonal antibody.
Western Blot: SATB1 Antibody (OTI13D6) [NBP1-47950]

Western Blot: SATB1 Antibody (OTI13D6) [NBP1-47950]

Western Blot: SATB1 Antibody (13D6) [NBP1-47950] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SATB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SATB1.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-SATB1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (OTI13D6) [NBP1-47950]

Immunohistochemistry-Paraffin: SATB1 Antibody (13D6) [NBP1-47950] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-SATB1 mouse monoclonal antibody.

Applications for SATB1 Antibody (OTI13D6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 4 using NBP1-47950 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SATB1

The protein binds to DNA at special AT-rich sequences at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA.

Long Name

SATB Homeobox 1

Alternate Names

DNA-binding protein SATB1, SATB homeobox 1, special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's), Special AT-rich sequence-binding protein 1

Entrez Gene IDs

6304 (Human)

Gene Symbol

SATB1

Additional SATB1 Products

Product Documents for SATB1 Antibody (OTI13D6)

Certificate of Analysis

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Product Specific Notices for SATB1 Antibody (OTI13D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SATB1 Antibody (OTI13D6) (1)

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    Application: Western Blot
    Verified Customer | Posted 05/22/2014

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