SATB2 Antibody (CL0320)

Novus Biologicals | Catalog # NBP3-52103

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL0320
View all SATB2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q9UPW6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SATB2 Antibody (CL0320)

SATB2 Antibody (CL0320) Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Staining of rat hippocampus shows no positivity in neurons in the dentate gyrus as expected.
SATB2 Antibody (CL0320) Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
SATB2 Antibody (CL0320) Western Blot: SATB2 Antibody (CL0320) [NBP3-52103]

Western Blot: SATB2 Antibody (CL0320) [NBP3-52103]

Lane 1: Marker [kDa]
Lane 2: Negative control (vector only transfected HEK293T lysate)�
Lane 3: SATB2 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414656)
SATB2 Antibody (CL0320) Western Blot: SATB2 Antibody (CL0320) [NBP3-52103]

Western Blot: SATB2 Antibody (CL0320) [NBP3-52103]

Lane 1: Marker [kDa]
Lane 2: Human cell line HEL
SATB2 Antibody (CL0320) Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Staining of human liver shows no positivity in hepatocytes as expected.
SATB2 Antibody (CL0320) Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
SATB2 Antibody (CL0320) Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Staining of rat hippocampus shows moderate to strong nuclear positivity in CA1 layer neurons.
SATB2 Antibody (CL0320) Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Analysis in human rectum and liver tissues. Corresponding SATB2 RNA-seq data are presented for the same tissues.
SATB2 Antibody (CL0320) Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Immunohistochemistry: SATB2 Antibody (CL0320) [NBP3-52103]

Staining of human rectum shows strong nuclear positivity in glandular cells.
SATB2 Antibody (CL0320) Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0320) [NBP3-52103]

Immunocytochemistry/ Immunofluorescence: SATB2 Antibody (CL0320) [NBP3-52103]

Staining of U-138 MG cells showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Applications for SATB2 Antibody (CL0320)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SATB2

SATB2 binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and

Alternate Names

DNA-binding protein SATB2, FLJ32076, KIAA1034FLJ21474, MGC119474, MGC119477, SATB family member 2, SATB homeobox 2, Special AT-rich sequence-binding protein 2

Gene Symbol

SATB2

Additional SATB2 Products

Product Documents for SATB2 Antibody (CL0320)

Certificate of Analysis

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Product Specific Notices for SATB2 Antibody (CL0320)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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