SCAMP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89552

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SCAMP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DPVDVNPFQDPSVTQLTNAPQGGLAEFNPFSETNAATTVPVTQLPGSSQPAVLQPSVEPTQPTPQAVVSAAQAGLLRQQEELDRKAAELERKERELQNTVANLHV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCAMP2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCAMP2 Antibody [NBP1-89552]

Immunocytochemistry/ Immunofluorescence: SCAMP2 Antibody [NBP1-89552]

Immunocytochemistry/Immunofluorescence: SCAMP2 Antibody [NBP1-89552] - Staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SCAMP2 Antibody [NBP1-89552]

Immunohistochemistry-Paraffin: SCAMP2 Antibody [NBP1-89552]

Immunohistochemistry-Paraffin: SCAMP2 Antibody [NBP1-89552] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Western Blot: SCAMP2 Antibody [NBP1-89552]

Western Blot: SCAMP2 Antibody [NBP1-89552]

Western Blot: SCAMP2 Antibody [NBP1-89552] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
SCAMP2 Antibody - BSA Free Western Blot: SCAMP2 Antibody - BSA Free [NBP1-89552]

Western Blot: SCAMP2 Antibody - BSA Free [NBP1-89552]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Applications for SCAMP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCAMP2

Secretory carrier membrane proteins (SCAMPs) are proteins that are components of post-Golgi membranes. These proteins are implicated to function in membrane trafficking. In fibroblasts, SCAMPs are concentrated in compartments that are involved in the recycling of cell surface receptors and endocytosis. In neurons, SCAMPs are associated with synaptic vesicles, secretion granules and transporter vesicles. SCAMPs are composed of four central transmembrane regions and a cytoplasmic tail. Of the five known SCAMPs, SCAMPs 1-3 contain cytoplasmic N-terminal regions with NPF repeats. NPF repeats are found to interact with EH domain proteins that function in budding of transport vesicles from the plasma membrane or the Golgi complex. SCAMPs 4 and 5 lack the N-terminal NPF repeats. SCAMPs 1-4 are all ubiquitously coexpressed while SCAMP 5 is only detectable in the brain. Studies show that SCAMP 5 is expressed late in development which is coincident with expansion of mature synapses.

Alternate Names

secretory carrier membrane protein 2secretory carrier-associated membrane protein 2

Gene Symbol

SCAMP2

Additional SCAMP2 Products

Product Documents for SCAMP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SCAMP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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