SCARA5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77050

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SCARA5 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SCARA5. The immunogen is located within amino acids 350 - 400 of SCARA5. Amino Acid Squence: KGDRGPKGEKGEKGDRA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Chicken (86%), Rat (80%)

Specificity

At least four isoforms of SCARA5 are known to exist; this antibody will detect all four isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCARA5 Antibody - BSA Free

Western Blot: SCARA5 AntibodyBSA Free [NBP1-77050]

Western Blot: SCARA5 AntibodyBSA Free [NBP1-77050]

Western Blot: SCARA5 Antibody [NBP1-77050] - Human liver tissue lysate with SCARA5 antibody at 1 ug/mL.
SCARA5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCARA5 Antibody - BSA Free [NBP1-77050] -

Immunocytochemistry/ Immunofluorescence: SCARA5 Antibody - BSA Free [NBP1-77050] - Immunofluorescence of SCARA5 in HepG2 cells with SCARA5 antibody at 20 u/mL.
SCARA5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCARA5 Antibody - BSA Free [NBP1-77050] -

Immunocytochemistry/ Immunofluorescence: SCARA5 Antibody - BSA Free [NBP1-77050] - Immunocytochemistry of SCARA5 in HepG2 cells with SCARA5 antibody at 2.5 u/mL.

Applications for SCARA5 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2.5-20 ug/ml

Western Blot

1 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID: 29461673).

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCARA5

Scavenger receptor class A member 5 (SCARA5), also known as TESR, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARA5 is a cell-surface receptor that contains that collagen-like domain and N-terminal scavenger receptor cysteine rich domain typical of the class A scavenger receptor subfamily. Unlike other class A receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery. At least four isoforms of SCARB1 are known to exist.

Long Name

Scavenger Receptor Class A Member 5

Alternate Names

Tesr

Gene Symbol

SCARA5

UniProt

NP_776194 (Human)

Additional SCARA5 Products

Product Documents for SCARA5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SCARA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Primordial and Mature Germ Cells

Citations for SCARA5 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SCARA5 Antibody - BSA Free

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  • Q: We are trying to detect chicken SCARA5 protein by western blot using NBP1-77050 antibody (Antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SCARA5). I am wondering whether the antibody can detect the chicken SCARA5 protein based on homology between human and chicken. Can I get AA sequences of the antigenic peptide?

    A: I have now heard back from the lab in response to your enquiry. Although they cannot disclose the amino acid sequence of the antigenic peptide, they have analyzed how the immunogen compares to the equivalent sequence in chicken, and there is 70% homology. Unfortunately, based on this percentage and distribution of homologous amino acids, it is difficult to predict whether the antibody will be able to detect the chicken SCARA5 protein.

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