SCP1 Antibody - BSA Free

Novus Biologicals | Catalog # NB300-228

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SCP1 Primary Antibodies »

Product Specifications

Immunogen

A C-terminal synthetic peptide made to the human SCP1 protein sequence. [UniProt# Q15431]

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-228]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [NB300-228]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).
Simple Western: SCP1 Antibody [NB300-228]

Simple Western: SCP1 Antibody [NB300-228]

Simple Western: SCP1 Antibody [NB300-228] - Lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody

Applications for SCP1 Antibody - BSA Free

Application
Recommended Usage

Immunoblotting

reported in scientific literature (PMID 27431324)

Immunocytochemistry/ Immunofluorescence

1:300-1:750

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 26858254)

Simple Western

1:200

Western Blot

reported in scientific literature (PMID 23843895)
Application Notes

This SCP1 antibody does not appear to work in Immunohistochemistry on paraffin-embedded sections. *The investigator should determine the optimal working dilution for a specific application.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Testis and Mouse Testis lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:200. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 1 review rated 1 using NB300-228 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SCP1

SCP1 (synaptonemal complex protein 1), also called cancer/testis antigen 8 (CT8), belongs to a growing family of cancer/testis antigens (CTA) which includes several proteins (MAGE, GAGE/PAGE/XAGE, NY-ESO-1/LAGE-1, SSX, SPANX, TRAG-3, BAGE, OY-TES-1, CT17, NY-BR-3 and SCP-1) that are not expressed in normal tissues except for testis and, in some cases, placenta. SCP1 is found in a complex with SYCE1 and SYCE2, and is a major component of the transverse filaments of synaptonemal complexes. SCP-1 is established to be expressed selectively during the meiotic prophase of spermatocytes formation and is involved in the pairing of homologous chromosomes during meiosis I. SCP1 is localized in tripartite segments of synaptonemal complexes, between lateral elements in the nucleus, and is found only where the chromosome cores are synapsed. Its N-terminus is found towards the center, whereas, the C-terminus extends well into the lateral domain of the synaptonemal complex. SCP-1 differs from other members of CTA family by its localization on chromosome 1 and it is frequently observed to have variable expression in neoplastic tissues/cell lines including malignant gliomas, breast, renal cell, ovarian, pancreatic and hepatocellular carcinomas. Because of exclusive expression of SCP1 in testis/placenta, it is emerging as an important target for cancer research and therapy.

Alternate Names

Cancer/testis antigen 8, CT8MGC104417, HOM-TES-14, SCP1SCP-1, synaptonemal complex protein 1

Gene Symbol

SYCP1

Additional SCP1 Products

Product Documents for SCP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SCP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SCP1 Antibody - BSA Free

Customer Reviews for SCP1 Antibody - BSA Free (1)

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  • SCP1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Induced pluripotent stem cells overexpressing SYCP1 and Meiocytes
    Species: Human
    Verified Customer | Posted 03/26/2024
    Human iPSCs overexpressing SYCP1, stained at 1:250 dilution of primary antibody.
    Stained at 1:250 dilution, using donkey anti-rabbit 488 secondary antibody. No signal was observed in human meiotic cells. I also tested this with human iPSCs overexpressing SYCP1 and also observed no signal.
    SCP1 Antibody - BSA Free NB300-228
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

View specific protocols for SCP1 Antibody - BSA Free (NB300-228):

SCP1 Antibody:
Immunofluorescence Procedure

1. Freshly prepared slides are soaked in 1X ADB for 75 minutes.

2. Primary antibodies are added concurrently (SCP1 and CDK2).

3. The primary antibodies are incubated overnight in a humid chamber (37 degrees Celsius).

4. The slides are washed for 40 minutes in 1X ADB.

5. The slides are detected with the appropriate secondary antibodies (RDAR for SCP1 and FDAM for CDK2).

6. The slides are incubated for 4 hours in a humid chamber (37 degrees Celsius).

7. The slides are washed for 20 minutes in 1X ADB, followed by 3 washes, 10 minutes each, in 1X PBS.

8. The slides are counterstained with DAPI.

9. Images are captured after allowing the slides to remain in the dark overnight at RT.

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FAQs

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