SCUBE2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-77186
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2. The immunogen is located within amino acids 480 - 530 of SCUBE2. Amino Acid Squence: CSPLRNKQQKSNDSAFGD
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SCUBE2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186] -
Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186] - Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 ug/mL.Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186] -
Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186] - Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 ug/mL.Western Blot: SCUBE2 Antibody - BSA Free [NBP1-77186] -
Western Blot: SCUBE2 Antibody - BSA Free [NBP1-77186] - Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Applications for SCUBE2 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SCUBE2
Alternate Names
Cegb1, Cegf1, CEGP1, FLJ16792, FLJ35234, MGC133057, Protein CEGP1, signal peptide, CUB and EGF-like domain-containing protein 2, signal peptide, CUB domain, EGF-like 2
Gene Symbol
SCUBE2
UniProt
Additional SCUBE2 Products
Product Documents for SCUBE2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SCUBE2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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