SDHA Antibody (GT20710) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13522

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Chicken (97%), Xenopus (94%), Zebrafish (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GT20710

Format

Azide and BSA Free
View all SDHA Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.

Reactivity Notes

Drosophila (87%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SDHA Antibody (GT20710) - Azide and BSA Free

Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:1000.
Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Rat colon. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.
Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000.
Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522]

Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Mouse kidney. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522]

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - Mouse liver. SDHA stained by SDHA antibody [20710] (NBP3-13522) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SDHA Antibody (GT20710)

Western Blot: SDHA Antibody (GT20710) [NBP3-13522] -

Western Blot: SDHA Antibody (GT20710) [NBP3-13522] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (NBP3-13522) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
SDHA Antibody (GT20710)

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] -

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:500.
Red: MitoTracker® Red CMXRos, a mitochondria marker.
Blue: Fluoroshield with DAPI.
SDHA Antibody (GT20710)

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] -

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] - SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SDHA Antibody (GT20710) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SDHA Antibody (GT20710) [NBP3-13522] -

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
SDHA Antibody (GT20710) - Azide and BSA Free

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] -

SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SDHA Antibody (GT20710) - Azide and BSA Free

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] -

SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SDHA Antibody (GT20710) - Azide and BSA Free

Immunohistochemistry-Paraffin: SDHA Antibody (GT20710) [NBP3-13522] -

SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SDHA stained by SDHA antibody [GT20710] (NBP3-13522) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for SDHA Antibody (GT20710) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDHA

The SDHA gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations

Long Name

SDHA

Alternate Names

CMD1GG, MC2DN1, NDAXOA, PGL5, PPGL5, SDH1, SDH2, SDHF

Gene Symbol

SDHA

Additional SDHA Products

Product Documents for SDHA Antibody (GT20710) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SDHA Antibody (GT20710) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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