SEC22B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-98766

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

Azide and BSA Free
View all SEC22B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from human SEC22B conjugated to blue carrier protein was used as the antigen.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for SEC22B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Frozen

10-50 ug/ml

Immunohistochemistry-Paraffin

10-50 ug/ml

Western Blot

10-50 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 27319744).

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SEC22B

The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins. There is evidence for use of multiple polyadenylation sites for the transcript. FUNCTION: SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER. SUBUNIT: Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex. SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein. Golgi apparatus membrane; Single-pass type IV membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Alternate Names

ERS24, ERS-24sec22b, SEC22 vesicle trafficking protein homolog B (S. cerevisiae), SEC22 vesicle trafficking protein homolog B (S. cerevisiae) (gene/pseudogene), SEC22 vesicle trafficking protein-like 1, SEC22 vesicle trafficking protein-like 1 (S. cerevisiae), SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, SEC22, vesicle trafficking protein (S. cerevisiae)-like 1, SEC22L1ER-Golgi SNARE of 24 kDa, vesicle-trafficking protein SEC22b

Entrez Gene IDs

9554 (Human); 20333 (Mouse); 310710 (Rat)

Gene Symbol

SEC22B

UniProt

O75396 (Human)

Additional SEC22B Products

Product Documents for SEC22B Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SEC22B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SEC22B Antibody - Azide and BSA Free

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Protocols

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FAQs

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