SEC31A Antibody (5D12C6) - BSA Free

Novus Biologicals | Catalog # NBP2-61870

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D12C6

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human SEC31A (AA: 429-571) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SEC31A Antibody (5D12C6) - BSA Free

Western Blot: SEC31A Antibody (5D12C6)BSA Free [NBP2-61870]

Western Blot: SEC31A Antibody (5D12C6)BSA Free [NBP2-61870]

Western Blot: SEC31A Antibody (5D12C6) [NBP2-61870] - Analysis using SEC31A mAb against human SEC31A (AA: 429-571) recombinant protein. (Expected MW is 41.8 kDa)
Immunohistochemistry: SEC31A Antibody (5D12C6) - BSA Free [NBP2-61870]

Immunohistochemistry: SEC31A Antibody (5D12C6) - BSA Free [NBP2-61870]

Immunohistochemistry: SEC31A Antibody (5D12C6) [NBP2-61870] - Analysis of paraffin-embedded ovarian cancer tissues using SEC31A mouse mAb with DAB staining.
Western Blot: SEC31A Antibody (5D12C6)BSA Free [NBP2-61870]

Western Blot: SEC31A Antibody (5D12C6)BSA Free [NBP2-61870]

Western Blot: SEC31A Antibody (5D12C6) [NBP2-61870] - Analysis using SEC31A mAb against HEK293 (1) and SEC31A (AA: 429-571)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: SEC31A Antibody (5D12C6) - BSA Free [NBP2-61870]

ELISA: SEC31A Antibody (5D12C6) - BSA Free [NBP2-61870]

ELISA: SEC31A Antibody (5D12C6) [NBP2-61870] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for SEC31A Antibody (5D12C6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SEC31A

The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.;

Alternate Names

ABP125ABP130KIAA0905Web1-like protein, DKFZp686N07171, HSPC334, MGC90305, protein transport protein Sec31A, protein-transport protein SEC31, SEC31 homolog A (S. cerevisiae), SEC31L1, SEC31-like 1, SEC31-like 1 (S. cerevisiae), SEC31-like protein 1, SEC31-related protein A, yeast Sec31p homolog

Gene Symbol

SEC31A

Additional SEC31A Products

Product Documents for SEC31A Antibody (5D12C6) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SEC31A Antibody (5D12C6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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