Sec8 Antibody

Novus Biologicals | Catalog # NBP1-83944

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Sec8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids:RDHLRGQAGFFSTPGGASRDGSGPGEEGKQRTLTTLLEKVEGCRHLLETPGQYLVYNGDLVEYDADHMAQLQRVHGFLMNDCLLVATWLPQRRGMYRYNAL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sec8 Antibody

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944]

Immunohistochemistry-Paraffin: Sec8 Antibody [NBP1-83944] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.
Sec8 Antibody Immunocytochemistry/ Immunofluorescence: Sec8 Antibody [NBP1-83944]

Immunocytochemistry/ Immunofluorescence: Sec8 Antibody [NBP1-83944]

Staining of human cell line U-2 OS shows localization to cytosol.

Applications for Sec8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sec8

Sec8 is encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

exocyst complex component 4, KIAA1699SEC8L1, MGC27170, REC8, SEC8Exocyst complex component Sec8, SEC8-like 1, SEC8-like 1 (S. cerevisiae), Sec8p

Gene Symbol

EXOC4

Additional Sec8 Products

Product Documents for Sec8 Antibody

Certificate of Analysis

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Product Specific Notices for Sec8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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