Seladin 1 Antibody (HL3253)

Novus Biologicals | Catalog # NBP3-48781

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3253
View all Seladin 1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Seladin 1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Seladin 1 Antibody (HL3253)

Seladin 1 Antibody (HL3253)

Western Blot: Seladin 1 Antibody (HL3253) [NBP3-48781] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Seladin 1 Antibody (HL3253)

Western Blot: Seladin 1 Antibody (HL3253) [NBP3-48781] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Seladin 1 Antibody (HL3253)

Immunocytochemistry/ Immunofluorescence: Seladin 1 Antibody (HL3253) [NBP3-48781] -

Seladin 1 antibody [HL3253] detects Seladin 1 protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: Seladin 1 stained by Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Seladin 1 Antibody (HL3253)

Western Blot: Seladin 1 Antibody (HL3253) [NBP3-48781] -

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Seladin 1 antibody [HL3253] (NBP3-48781) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Seladin 1 Antibody (HL3253)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Seladin 1

Seladin 1 encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.

Alternate Names

24-dehydrocholesterol reductaseNbla03646, DCE, desmosterol-to-cholesterol enzyme, Diminuto/dwarf1 homolog, EC 1.3.1.72, KIAA0018SELADIN1, seladin 1,3-beta-hydroxysterol delta-24-reductase, seladin-1, selective AD indicator 1,3 beta-hydroxysterol delta 24-reductase

Gene Symbol

DHCR24

Additional Seladin 1 Products

Product Documents for Seladin 1 Antibody (HL3253)

Certificate of Analysis

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Download SDS

Product Specific Notices for Seladin 1 Antibody (HL3253)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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