SERCA2 ATPase Antibody (2A7-A1)

Novus Biologicals | Catalog # NB300-581

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Amphibian, Canine, Guinea Pig, Rabbit, Sheep

Cited:

Mouse, Rat, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2A7-A1
View all SERCA2 ATPase Primary Antibodies »

Product Specifications

Immunogen

Purified canine cardiac sarcoplasmic reticulum vesicles.

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 21289077). Amphibian reactivity reported in scientific literature (PMID: 12022876). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects sarcoplasmic or endoplasmic reticulum calcium (SERCA) 2 ATPase. NB300-581 recognizes the SERCA2a and SERCA2b isoforms identically. However, the antibody does not recognize the skeletal muscle (SERCA1) or housekeeping isoforms (SERCA3).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SERCA2 ATPase Antibody (2A7-A1)

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human skeletal muscle tissues.
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/ Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581]

Immunohistochemistry-Paraffin: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - Both normal and cancer biopsies of deparaffinized Human liver tissues.

Applications for SERCA2 ATPase Antibody (2A7-A1)

Application
Recommended Usage

Flow Cytometry

1 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1 ug/mL

Immunoprecipitation

1:10 - 1:500

Simple Western

1:10 - 1:250

Western Blot

1:1000
Application Notes
WB: Detects an approx. 110 kDa protein representing SERCA2 ATPase in rat cardiac tissue.

Reviewed Applications

Read 2 reviews rated 3 using NB300-581 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SERCA2 ATPase

ATP dependent calcium pumps are responsible in part for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are comprised of a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific isoform expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

Long Name

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2

Alternate Names

ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+)-ATPase 2

Entrez Gene IDs

488 (Human); 11938 (Mouse); 29693 (Rat); 403878 (Canine); 100038308 (Rabbit)

Gene Symbol

ATP2A2

UniProt

O46674 (Canine)

Additional SERCA2 ATPase Products

Product Documents for SERCA2 ATPase Antibody (2A7-A1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SERCA2 ATPase Antibody (2A7-A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SERCA2 ATPase Antibody (2A7-A1)

Customer Reviews for SERCA2 ATPase Antibody (2A7-A1) (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • SERCA2 ATPase Antibody (2A7-A1)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: cardiac tissue
    Species: Rat
    Verified Customer | Posted 12/16/2020
    Rat model of heart failure post-myocardial infarction. Non-specific staining in infarct region and healthy myocardium due to high cross-reactivity. 10X magnification
    1:900 dilution Nuclear counterstain
    SERCA2 ATPase Antibody (2A7-A1) NB300-581
  • SERCA2 ATPase Antibody (2A7-A1)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: hela cell
    Species: Human
    Verified Customer | Posted 08/01/2018
    HeLa cell Primary Ab: 1:100 Secondary Ab: Alexa fluor 568 conjugated Goat anti-Mouse IgG 1:100
    SERCA2 ATPase Antibody (2A7-A1) NB300-581

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