Serpin A12 Antibody (VP63) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80041
![Western Blot: Serpin A12 Antibody (VP63)Azide and BSA Free [NBP2-80041]](https://resources.rndsystems.com/images/products/Serpin-A12-Antibody-VP63-Western-Blot-NBP2-80041-img0003.jpg "Western Blot: Serpin A12 Antibody (VP63)Azide and BSA Free [NBP2-80041]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # VP63
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant human Serpin A12.
Specificity
Recognizes human Serpin A12. Detects a band of ~48kDa by Western blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Serpin A12 Antibody (VP63) - Azide and BSA Free
Western Blot: Serpin A12 Antibody (VP63)Azide and BSA Free [NBP2-80041]
Western Blot: Serpin A12 Antibody (VP63) [NBP2-80041] - Western blot analysis using anti-Serpin A12 (human), mAb (VP63) at 1:1000 dilution. 1: Human vaspin (His-tagged). 2: Human vaspin (FLAG-tagged). 3: Mouse IL-33 (His-tagged) (negative control).![Immunohistochemistry: Serpin A12 Antibody (VP63) - Azide and BSA Free [NBP2-80041]](https://resources.rndsystems.com/images/products/Serpin-A12-Antibody-VP63-Immunohistochemistry-NBP2-80041-img0002.jpg "Immunohistochemistry: Serpin A12 Antibody (VP63) - Azide and BSA Free [NBP2-80041]")
Immunohistochemistry: Serpin A12 Antibody (VP63) - Azide and BSA Free [NBP2-80041]
Immunohistochemistry: Serpin A12 Antibody (VP63) [NBP2-80041] - Immunohistochemical staining of vaspin with anti-Serpin A12 (human), mAb (VP63) in healthy skin (A). Arrow - stronger staining in Stratum granulosum. Negative control for vaspin in healthy skin (B). Blue hematoxylin stained cell nuclei.![Immunohistochemistry: Serpin A12 Antibody (VP63) - Azide and BSA Free [NBP2-80041]](https://resources.rndsystems.com/images/products/Serpin-A12-Antibody-VP63-Immunohistochemistry-NBP2-80041-img0001.jpg "Immunohistochemistry: Serpin A12 Antibody (VP63) - Azide and BSA Free [NBP2-80041]")
Immunohistochemistry: Serpin A12 Antibody (VP63) - Azide and BSA Free [NBP2-80041]
Immunohistochemistry: Serpin A12 Antibody (VP63) [NBP2-80041] - Immunohistochemical staining of vaspin with anti-Serpin A12 (human), mAb (VP63) in psoriatic skin lesions (A). Negative control for vaspin in psoriatic skin lesions (B). Blue hematoxylin stained cell nuclei.Applications for Serpin A12 Antibody (VP63) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:2000 - 1:5000
Immunohistochemistry-Frozen
1:100
Western Blot
1:2000 - 1:5000
Application Notes
Western blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST
with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom
temperature.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um-filtered solution in PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Serpin A12
Alternate Names
OL-64, Vaspin
Gene Symbol
SERPINA12
Additional Serpin A12 Products
Product Documents for Serpin A12 Antibody (VP63) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Serpin A12 Antibody (VP63) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
