Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C5

Format

BSA Free
View all Serpin E1/PAI-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Serpin E1/PAI-1 (24-402aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Novus Biologicals Mouse Serpin E1/PAI-1 Antibody (1C5) - BSA Free (NBP2-59495) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Serpin E1/PAI-1 Antibody (1C5) - BSA Free

Western Blot: Serpin E1/PAI-1 Antibody (1C5)BSA Free [NBP2-59495]

Western Blot: Serpin E1/PAI-1 Antibody (1C5)BSA Free [NBP2-59495]

Western Blot: Serpin E1/PAI-1 Antibody (1C5) [NBP2-59495] - Lane 1: 293T cell lysates, Lane 2: PAI1 transfected 293T cell lysates
Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody (1C5) - BSA Free [NBP2-59495]

Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody (1C5) - BSA Free [NBP2-59495]

Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody (1C5) [NBP2-59495] - Analysis of PAI1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PAI1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

Applications for Serpin E1/PAI-1 Antibody (1C5) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serpin E1/PAI-1

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Gene Symbol

SERPINE1

Additional Serpin E1/PAI-1 Products

Product Documents for Serpin E1/PAI-1 Antibody (1C5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Serpin E1/PAI-1 Antibody (1C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail