SET Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-33713
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human SET. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Endoplasmic reticulum, Nucleus, nucleoplasm
Specificity
Antibody reactive against recombinant protein, detects immunogen. Further validation in progress.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit SET Antibody - BSA Free (NBP1-33713) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-SET Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for SET Antibody - BSA Free
Western Blot: SET Antibody [NBP1-33713]
Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw 264.7 whole cell lysate/extract. G. 30 ug C2Cl2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000Western Blot: SET Antibody [NBP1-33713]
Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by Western blot analysis. A. 30 ug A431 whole cell lysate/extract whole cell lysate/extract. B. 30 ug H1299 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug Molt-4 whole cell lysate/extract. F. 30 ug Raji whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000 SET antibody detects SET protein by Western blot analysis.Observed SET protein is larger than the predicted M.W., possibly due to post-translationalWestern Blot: SET Antibody [NBP1-33713]
Western Blot: SET Antibody [NBP1-33713] - SET antibody detects SET protein by western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. SET antibody dilution: 1:1000.Immunoprecipitation: SET Antibody [NBP1-33713]
Immunoprecipitation: SET Antibody [NBP1-33713] - SET antibody immunoprecipitates SET protein in IP experiments. IP samples: HeLa whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of SET protein by 4 ug SET antibody. 10 % SDS-PAGE. The immunoprecipitated SET protein was detected by SET antibody diluted at 1:500.Western Blot: SET Antibody [NBP1-33713] -
Western Blot: SET Antibody [NBP1-33713] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SET antibody (NBP1-33713) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: SET Antibody [NBP1-33713] -
SET antibody detects SET protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SET protein stained by SET antibody (NBP1-33713) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.
Western Blot: SET Antibody - BSA Free [NBP1-33713] -
Raddeanin A‐treated NK cells enhance apoptosis in FaDu cells. (A, B) Mitochondrial membrane potential assay and (C, D) annexin V/PI stain assay of FaDu cells were measured after Raddeanin A treatment by Muse Cell Analyser. Quantitative data were analysed by Muse Cell Software V1.4.0.0. (E) Fas protein levels of Raddeanin A‐induced apoptosis. FaDu cells were cocultured with KHYG‐1 cells at an effector: Target ratio of 6:1 And then treated with Raddeanin A for 24 h. (F) FaDu cells were treated with Raddeanin A for 24 h. Cell viability was assessed using WST‐8 assays. (G, H) Protein levels of Raddeanin A‐induced apoptosis. FaDu cells were cocultured with KHYG‐1 cells at an effector: Target ratio of 6:1 and then treated with Raddeanin A for 24 h. ImageJ was used for protein quantification; the levels of all proteins were normalized to that of beta ‐Actin. Data were presented as the mean +/- standard deviation (n = 3) of three independent experiments. *p < 0.05 compare with control; # p < 0.05 compare with cotreatment control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39175122), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: SET Antibody - BSA Free [NBP1-33713] -
Raddeanin A‐treated NK cells enhance apoptosis in K562 cells. Raddeanin A induced apoptosis in cocultured K562 cells. KHYG‐1 cells were pretreated with Raddeanin A for 24 h before being transferred to a co‐culture system. (A and B) Mitochondrial membrane potential assay and (C and D) annexin V/PI stain assay of K562 cells were measured after Raddeanin A treatment by Muse Cell Analyser. Quantitative data were analysed by Muse Cell Software V1.4.0.0. (E) Fas protein levels of Raddeanin A‐induced apoptosis. K562 cells were cocultured with KHYG‐1 cells at an effector: Target ratio of 6:1 And then treated with Raddeanin A for 24 h. (F) K562 cells were treated with Raddeanin A for 24 h. Cell viability was assessed using WST‐8 assays. (G, H) Protein levels of Raddeanin A‐induced apoptosis. K562 cells were cocultured with KHYG‐1 cells at an effector: Target ratio of 6:1 And then treated with Raddeanin A for 24 h. ImageJ was used for protein quantification; the levels of all proteins were normalized to that of beta ‐Actin. Data were presented as the mean +/- standard deviation (n = 3) of three independent experiments. *p < 0.05 compare with control; # p < 0.05 compare with cotreatment control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39175122), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Western Blot: SET Antibody - BSA Free [NBP1-33713] -
Raddeanin A‐treated NK cells enhance apoptosis in NPC‐039 cells. (A, B) Mitochondrial membrane potential assay and (C, D) annexin V/PI stain assay of NPC‐039 cells were measured after Raddeanin A treatment by Muse Cell Analyser. Quantitative data were analysed by Muse Cell Software V1.4.0.0. (E) Fas protein levels of Raddeanin A‐induced apoptosis. NPC‐039 cells were cocultured with KHYG‐1 cells at an effector: Target ratio of 6:1 And then treated with Raddeanin A for 24 h. (F) NPC‐039 cells were treated with Raddeanin A for 24 h. Cell viability was assessed using WST‐8 assays. (G, H) Protein levels of Raddeanin A‐induced apoptosis. NPC‐039 cells were cocultured with KHYG‐1 cells at an effector: Target ratio of 6:1 And then treated with Raddeanin A for 24 h. ImageJ was used for protein quantification; the levels of all proteins were normalized to that of beta ‐Actin. Data were presented as the mean +/- standard deviation (n = 3) of three independent experiments. *p < 0.05 compare with control; # p < 0.05 compare with cotreatment control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39175122), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SET Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SET
Alternate Names
2PP2A, HLA-DR-associated protein II, I2PP2A, I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, inhibitor-2 of protein phosphatase-2A, IPP2A2, PHAPIITAF-I, Phosphatase 2A inhibitor I2PP2A, protein phosphatase type 2A inhibitor, protein SET, SET nuclear oncogene, SET translocation (myeloid leukemia-associated), TAF-IBETA, Template-activating factor I, Template-Activating Factor-I, chromatin remodelling factor
Entrez Gene IDs
6418 (Human)
Gene Symbol
SET
UniProt
Additional SET Products
Product Documents for SET Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SET Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for SET Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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