SETDB2 Antibody

Novus Biologicals | Catalog # NB100-1137

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SETDB2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence GEKNGDAKTFWME-C corresponding to N-Terminus according to NP_114121.2, NP_001153780.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137]

Western Blot: SETDB2 Antibody [NB100-1137]

Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Nuclear HeLa lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NB100-1137]

Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NB100-1137]

Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - Human dermal microvascular endothelial cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% TritonX-100 and immunostained with NB100-1137 (1:100 dilution). Secondary antibody conjugated to Alexafluor 488. Image submitted by a verified customer review.
Chromatin Immunoprecipitation (ChIP): SETDB2 Antibody [NB100-1137] - Human B-cell nuclear extract (500 ug). Gene of interest promoter primers were used for amplification to detect ChIP efficiency to IgG control. Image submitted by a verified customer review.
Western Blot: SETDB2 Antibody [NB100-1137]

Western Blot: SETDB2 Antibody [NB100-1137]

Western Blot: SETDB2 Antibody [NB100-1137] - RL human non-Hodgkin's lymphoma B cell line whole cell lysate (WCL). Single band around correct MW is specific to the target protein SETDB2. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NB100-1137]

Immunocytochemistry/ Immunofluorescence: SETDB2 Antibody [NB100-1137]

Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - SETDB2 in human induced Pluripotent Stem Cells (iPSCs) using anti-SETDB2 antibody. Image submitted by a verified customer review.
SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137] -

Demonstration of IFI16’s interaction with specific H3K9 MTases in KSHV latently infected PEL (BCBL-1 and BC-3) cells and in uninfected control BJAB cells.(A) Nuclear fractions were isolated from latently infected cells and uninfected BJAB cells and treated with Benzonase. IPs were performed using anti-IFI16 mAb and LANA mAb and WBs were performed. (B) To confirm IFI16’s and LANA’s interaction with H3K9 MTases, IPs were done with Abs against the H3K9 MTases and blotted for the corresponding MTase, IFI16, LANA and HP1 alpha (heterochromatin protein 1 alpha ). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31682228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137] -

Demonstration of IFI16’s interaction and recruitment of specific H3K9 MTases during de novo KSHV infection.(A) TIME cells either left uninfected or infected with KSHV for 6 or 24 hr were IPed with anti-IFI16 antibodies and western blotted for the indicated proteins. (B) To confirm IFI16’s interaction with H3K9 MTases, TIME cells were infected as in (A) and IPed with antibodies against the MTases and blotted for the corresponding MTase and IFI16. (C) 293 T cells lacking IFI16 transfected with control plasmid or His-IFI16 expressing plasmid for 72 hr were utilized for His-tag pulldown using HisPur cobalt resin. Inputs and elutions were blotted for the indicated proteins. (D) 293 T cells transfected with control plasmid, IFI16 expressing plasmid or LANA expressing plasmid for 72 hr were IPed with anti-IFI16 mAb or LANA mAb. Inputs and elutions were blotted for the indicated proteins. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31682228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137] -

Demonstration of IFI16’s interaction and recruitment of specific H3K9 MTases during de novo KSHV infection.(A) TIME cells either left uninfected or infected with KSHV for 6 or 24 hr were IPed with anti-IFI16 antibodies and western blotted for the indicated proteins. (B) To confirm IFI16’s interaction with H3K9 MTases, TIME cells were infected as in (A) and IPed with antibodies against the MTases and blotted for the corresponding MTase and IFI16. (C) 293 T cells lacking IFI16 transfected with control plasmid or His-IFI16 expressing plasmid for 72 hr were utilized for His-tag pulldown using HisPur cobalt resin. Inputs and elutions were blotted for the indicated proteins. (D) 293 T cells transfected with control plasmid, IFI16 expressing plasmid or LANA expressing plasmid for 72 hr were IPed with anti-IFI16 mAb or LANA mAb. Inputs and elutions were blotted for the indicated proteins. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31682228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137] -

Demonstration of IFI16’s interaction with specific H3K9 MTases in KSHV latently infected PEL (BCBL-1 and BC-3) cells and in uninfected control BJAB cells.(A) Nuclear fractions were isolated from latently infected cells and uninfected BJAB cells and treated with Benzonase. IPs were performed using anti-IFI16 mAb and LANA mAb and WBs were performed. (B) To confirm IFI16’s and LANA’s interaction with H3K9 MTases, IPs were done with Abs against the H3K9 MTases and blotted for the corresponding MTase, IFI16, LANA and HP1 alpha (heterochromatin protein 1 alpha ). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31682228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137] -

Effect of A366 on KSHV life cycle and the demonstration of IFI16’s association with cellular H3K9 methyltransferase(s) (H3K9 MTase) and recruitment of various H3K9 MTases to the KSHV genome during de novo infection.(A) MTT cell viability assay of BCBL-1 cells treated with the H3K9me3 specific chemical inhibitor A366 at different concentrations and different time points. (B) q-RT PCR (two-step, sybr Green) of KSHV mRNAs in BCBL-1 cells treated for 72 hr with either vehicle control DMSO or A366 (10 uM and 100 uM). (C) WB of different H3 methylations and IFI16 after A366 treatment of BCBL-1 cells. (D) H3K9 methyltransferase activity (ng/h/mg) assay. TIME cells were infected with KSHV for 6 or 24 hr followed by isolation of nuclear fraction, benzonase treatment and IP with anti-IFI16 or control IgG in the presence of benzonase using the catch and release method. Elution was performed under non-denaturing conditions to keep the associated H3K9 methyltransferase active. H3K9 methyltransferase activity was assayed in the eluate (Materials and methods). *, p<0.05; **, p<0.01; ***,<0.001; unpaired t-test. (E) TIME cells were infected with KSHV genome labeled with EdU or unlabeled control KSHV (100 DNA copies/cell) for 24 hr followed by EdU-KSHV genome pulldown using Click chemistry. The inputs and eluates were blotted for different H3K9 MTases. (F) TIME cells were infected with EdU-labeled KSHV as in (D) and stained using the Click-iT EdU Alexa Fluor 594 Imaging Kit (red). Subsequently, IFA was performed against different H3K9 MTases and colocalization of the IFA signal (green) with KSHV EdU-genome staining (red) resulting in yellow was evaluated (enlarged image, white arrows). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31682228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137] -

Demonstration of IFI16’s interaction and recruitment of specific H3K9 MTases during de novo KSHV infection.(A) TIME cells either left uninfected or infected with KSHV for 6 or 24 hr were IPed with anti-IFI16 antibodies and western blotted for the indicated proteins. (B) To confirm IFI16’s interaction with H3K9 MTases, TIME cells were infected as in (A) and IPed with antibodies against the MTases and blotted for the corresponding MTase and IFI16. (C) 293 T cells lacking IFI16 transfected with control plasmid or His-IFI16 expressing plasmid for 72 hr were utilized for His-tag pulldown using HisPur cobalt resin. Inputs and elutions were blotted for the indicated proteins. (D) 293 T cells transfected with control plasmid, IFI16 expressing plasmid or LANA expressing plasmid for 72 hr were IPed with anti-IFI16 mAb or LANA mAb. Inputs and elutions were blotted for the indicated proteins. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31682228), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SETDB2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

1 - 3 ug/mL
Application Notes
Approx 85 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 81.9 kDa according to NP_114121.2). Not suitable on mouse heart and rat heart lysates. This SETDB2 antibody is validated for WB, CHIP, ICC/IF IP from verified customer reviews.

Reviewed Applications

Read 6 reviews rated 4 using NB100-1137 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SETDB2

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity.

Alternate Names

C13orf4, chromosome 13 open reading frame 4, chronic lymphocytic leukemia deletion region 8, Chronic lymphocytic leukemia deletion region gene 8 protein, CLLD8DKFZp761J1217, DKFZp586I0123, EC 2.1.1.43, histone-lysine N-methyltransferase SETDB2, KMT1FCLLL8, Lysine N-methyltransferase 1F, SET domain bifurcated 2, SET domain, bifurcated 2

Entrez Gene IDs

83852 (Human)

Gene Symbol

SETDB2

UniProt

NP_114121. (Human)

Additional SETDB2 Products

Product Documents for SETDB2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SETDB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SETDB2 Antibody

Customer Reviews for SETDB2 Antibody (6)

4 out of 5
6 Customer Ratings
5 Stars
17%
4 Stars
67%
3 Stars
17%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 6 reviews Showing All
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  • SETDB2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: THP1 cell line
    Species: Human
    Verified Customer | Posted 07/18/2018
    Thp1 whole cell lysate was immunobloted with SETDB2 antibody.
    SETDB2 Antibody NB100-1137
  • SETDB2 Antibody
    Name: Anonymous
    Application: Chromatin Immunoprecipitation
    Sample Tested: B cells
    Species: Human
    Verified Customer | Posted 06/22/2018
    ChIP was performed with 500 ug of Nuclear extract using 3 ug of NB100-1137. Gene of interest promoter primers was used to detect ChIP efficiency and compared to ChIP using control IgG.
    SETDB2 Antibody NB100-1137
  • SETDB2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human Dermal Microvascular Endothelial cells
    Species: Human
    Verified Customer | Posted 06/18/2018
    Human Dermal Microvascular Endothelial cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% TritonX-100 and immunostained with NB100-1137 (1:100 dilution). Secondary antibody conjugated to Alexafluor 488 was used.
    SETDB2 Antibody NB100-1137
  • SETDB2 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: RL human non-Hodgkin's lymphoma B cell line
    Species: Human
    Verified Customer | Posted 05/16/2018
    IP was performed on human non-Hodgkin's lymphoma B cell line with NB100-1137 and IgG as indicated. WB was performed with NB100-1137.
    SETDB2 Antibody NB100-1137
  • SETDB2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: RL human non-Hodgkin's lymphoma B cell line
    Species: Human
    Verified Customer | Posted 01/04/2018
    WCL, whole cell lysate.
    SETDB2 Antibody NB100-1137
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 02/25/2016
    iPS cells, nuclear staining
    SETDB2 Antibody NB100-1137

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