SF2 Antibody

Novus Biologicals | Catalog # NBP2-20324

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (98%), Porcine (100%), Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SF2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SF2. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%).

Localization

Cytoplasm; Nucleus speckle

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SF2 Antibody

Immunocytochemistry/ Immunofluorescence: SF2 Antibody [NBP2-20324]

Immunocytochemistry/ Immunofluorescence: SF2 Antibody [NBP2-20324]

Immunocytochemistry/Immunofluorescence: SF2 Antibody [NBP2-20324] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SF2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Western Blot: SF2 Antibody [NBP2-20324]

Western Blot: SF2 Antibody [NBP2-20324]

Western Blot: SF2 Antibody [NBP2-20324] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324]

Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324]

Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Antibody detects SFRS1 protein at nucleus on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. Antibody dilution: 1:500.
Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324]

Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324]

Immunohistochemistry-Paraffin: SF2 Antibody [NBP2-20324] - Paraffin-embedded mouse skin. SF2 antibody dilution: 1:500.
SF2 Antibody

Immunohistochemistry: SF2 Antibody [NBP2-20324] -

SF2 antibody detects SFRS1 protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
SF2 antibody (NBP2-20324) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
SF2 Antibody

Immunohistochemistry: SF2 Antibody [NBP2-20324] -

SF2 antibody detects SF2 protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SF2 antibody (NBP2-20324) dilution: 1:500.

Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
SF2 Antibody

Western Blot: SF2 Antibody [NBP2-20324] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (NBP2-20324) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SF2 Antibody

Western Blot: SF2 Antibody [NBP2-20324] -

Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SF2 antibody (NBP2-20324) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SF2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SF2

This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alternative-splicing factor 1, ASFpre-mRNA-splicing factor SF2, P33 subunit, MGC5228, serine/arginine-rich splicing factor 1, SF2FLJ53078, SF2p33, SFRS1splicing factor 2, Splicing factor, arginine/serine-rich 1ASF-1, SR splicing factor 1, SRp30a

Gene Symbol

SRSF1

UniProt

Q07955 (Human)

Additional SF2 Products

Product Documents for SF2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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