sFRP-5 Antibody - BSA Free

Novus Biologicals | Catalog # NB200-121

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all sFRP-5 Primary Antibodies »

Product Specifications

Reactivity Notes

Human.

Specificity

Reacts with residues 25-38 [APARCEEYDYYGWQ] of human SFRP5. 92% identity bovine. 85% identity rat. 78% identity mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for sFRP-5 Antibody - BSA Free

Western Blot: sFRP-5 AntibodyBSA Free [NB200-121]

Western Blot: sFRP-5 AntibodyBSA Free [NB200-121]

Western Blot: SFRP5 Antibody [NB200-121] - Analysis of human SFRP5 in pancreas lysate with anti SFRP5 at a 1:500 dilution.

Applications for sFRP-5 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500 -1:1000
Application Notes
Detects a 38kDa band by Western Blot. The investigator should determine the optimal dilutions.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: sFRP-5

Human secreted frizzled-related protein 5 (SFRP5), which appears to act by modulating Wnt signal transduction. Wnts have been implicated in embryonic induction, cell fate determination, control of cell polarity and malignant transformation.

Long Name

Secreted Frizzled Related Protein 5

Alternate Names

FRP1B, SARP3, sFRP5

Gene Symbol

SFRP5

Additional sFRP-5 Products

Product Documents for sFRP-5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for sFRP-5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Family
Wnt Inhibitors

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Protocols

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FAQs

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