SGK3 Antibody

Novus Biologicals | Catalog # NBP3-12271

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit
View all SGK3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 440-490 on human Sgk3 protein.

Clonality

Polyclonal

Host

Rabbit

Description

Novus Biologicals Rabbit SGK3 Antibody (NBP3-12271) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SGK3 Antibody

Western Blot: SGK3 Antibody [NBP3-12271]

Western Blot: SGK3 Antibody [NBP3-12271]

Western Blot: SGK3 Antibody [NBP3-12271] - Rat testis. Diluted 1:250 in DiluObuffer. Apparent MW is 58 KDa.
Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271]

Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271]

Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271] - Rat duodenum. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.
Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271]

Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271]

Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271] - Mouse Small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.
Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271]

Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271]

Immunohistochemistry-Paraffin: SGK3 Antibody [NBP3-12271] - Rat Duodenum. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10x magnification.

Applications for SGK3 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: SGK3

SGKL/SGK3, also known as CISK for cytokine-independent survival kinase, is a SGK type protein kinase. The protein contains a lipid-binding phox homology (PX) domain, which is required for targeting SGKL/SGK3 protein to the endosomal compartment. SGKL/SGK3 functions downstream of the phosphoinositide 3-kinase (PI3K) cascade and has been implicated in cell survival. The protein has been shown to be phosphorylated and activated by 3-phosphoinositide-dependent protein kinase 1 (PDK1), and to a lesser extent insulin-like growth factor-1, in vitro. Activated SGKL/SGK3 has been shown to phosphorylate glycogen synthase kinase 3 beta (GSK-3beta) in vitro. SGKL/SGK3 expression has been documented in many human tissues but was found to be most abundant in lung. ESTs have been isolated from human tissue libraries, including normal liver, breast and thyroid and cancerous spleen, ear and pancreas.

Long Name

Serum/Glucocorticoid Regulated Kinase 3

Alternate Names

CISK

Gene Symbol

SGK3

Additional SGK3 Products

Product Documents for SGK3 Antibody

Certificate of Analysis

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Product Specific Notices for SGK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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