SH3BP5L Antibody - BSA Free

Western Blot: SH3BP5L Antibody - BSA Free [NBP1-81381] -

Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean +/- SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. Approximate molecular weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with DAPI staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant.) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39753851), licensed under a CC-BY license. Not internally tested by Novus Biologicals.