SH3PX1 Antibody (OTI1B5)

Novus Biologicals | Catalog # NBP2-02609

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B5
View all SH3PX1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SNX9(NP_057308) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

66.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SH3PX1 Antibody (OTI1B5)

Western Blot: SH3PX1 Antibody (1B5) [NBP2-02609] Analysis of extracts (35ug) from 9 different cell lines by using anti-SH3PX1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SH3PX1.
Immunohistochemistry-Paraffin: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunohistochemistry-Paraffin: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Human prostate tissue using anti-SH3PX1 mouse monoclonal antibody.
Flow Cytometry: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Flow Cytometry: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Flow Cytometry: SH3PX1 Antibody (1B5) [NBP2-02609] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SH3PX1 antibody, and then analyzed by flow cytometry.
Western Blot: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Western Blot: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Western Blot: SH3PX1 Antibody (1B5) [NBP2-02609] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3PX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3PX1.
Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunocytochemistry/ Immunofluorescence: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunocytochemistry/Immunofluorescence: SH3PX1 Antibody (1B5) [NBP2-02609] - Immunofluorescent staining of HeLa cells using anti-SH3PX1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunohistochemistry-Paraffin: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SH3PX1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunohistochemistry-Paraffin: SH3PX1 Antibody (OTI1B5) [NBP2-02609]

Immunohistochemistry-Paraffin: SH3PX1 Antibody (1B5) [NBP2-02609] - Staining of paraffin-embedded Human colon tissue using anti-SH3PX1 mouse monoclonal antibody.

Applications for SH3PX1 Antibody (OTI1B5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SH3PX1

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but does contain a SH3 domain near its N-terminus. This protein interacts with the cytoplasmic domains of the precursor but not the processed forms of a disintegrin and metalloprotease domain 9 and 15. This protein binds the beta-appendage domain of adaptor protein 2 and may function to assist adaptor protein 2 in its role at the plasma membrane. This protein interacts with activated Cdc42-associated kinase-2 to regulate the degradation of epidermal growth factor receptor protein.

Alternate Names

MST155, MSTP155, Protein SDP1, SDP1, SH3 and PX domain-containing protein 1, SH3PX1WISP, SH3PXD3ASH3 and PX domain-containing protein SH3PX1, sorting nexin 9, sorting nexin-9, WiskSH3 and PX domain-containing protein 3A

Entrez Gene IDs

51429 (Human)

Gene Symbol

SNX9

Additional SH3PX1 Products

Product Documents for SH3PX1 Antibody (OTI1B5)

Certificate of Analysis

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Product Specific Notices for SH3PX1 Antibody (OTI1B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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