SHANK1 Antibody - BSA Free
Novus Biologicals | Catalog # NB300-167
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
Reactivity Notes
Human, rat and mouse. Other species have not been tested.
Specificity
Reacts with residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SHANK1 Antibody - BSA Free
Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]
SHANK1-Antibody-Immunohistochemistry-NB300-167-img0012.jpgImmunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.Western Blot: SHANK1 AntibodyBSA Free [NB300-167]
Western Blot: SHANK1 Antibody [NB300-167] - Shank1a in rat brain lysate using NB300-167 at 1:500 dilution.Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]
SHANK1-Antibody-Immunohistochemistry-NB300-167-img0013.jpgImmunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167]
SHANK1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-167-img0010.jpgImmunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]
SHANK1-Antibody-Immunohistochemistry-NB300-167-img0014.jpgImmunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167]
SHANK1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-167-img0011.jpgImmunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]
SHANK1-Antibody-Immunohistochemistry-NB300-167-img0017.jpgImmunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Retinal tissue using NB300-167 at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Top box: 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar = 300 μm. Bottom box: same stainings as top box but with the VGLUT2 antibody (white; red in merge). Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 μm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Top box: 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar = 300 μm. Bottom box: same stainings as top box but with the VGLUT2 antibody (white; red in merge). Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 μm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank puncta with VGLUT2 puncta in the VGLUT2-band nor in the intragranular mossy fibers of the dentate gyrus. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: dentate Gyrus = DG, containing stratum granulare = SG, stratum moleculare = SM, & polymorphic layer = PL; I. indicates uppermost row/figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge), & VGLUT2 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 μm), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the outer part of the SG (2, top row) & in the PL (1, bottom row) (scale bar = 5 μm). Red arrows indicate VGLUT2-band. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the stratum radiatum of the CA1. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA1 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR; I./II./III. indicates uppermost/middle/bottom figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers & the stratum moleculare of the dentate gyrus. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: dentate Gyrus = DG, containing stratum granulare = SG, stratum moleculare = SM, & polymorphic layer = PL; I. indicates uppermost row/figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 μm), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the PL (1, top row) & SM (3, bottom row) (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank immunoreactivity with VGLUT2 puncta in the VGLUT2-band of the stratum pyramidale nor in the mossy fibers of the CA3. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA3 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT2 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows further enlargements (indicated in upper row by white rectangles) in the lateral part of the SP (2, top row) & the SR (1, bottom row) (scale bar = 5 μm). Red arrows indicate VGLUT2-band. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank immunoreactivity with VGLUT1 in the stratum radiatum/stratum lucidum of the CA3 region. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA3 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SHANK1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:800
Immunohistochemistry
1:250-1:750
Immunohistochemistry-Paraffin
1:250-1:750
Western Blot
1:500-1:1000
Application Notes
This antibody is useful for Western blot, immunohistochemistry-Paraffin, and immunohistochemistry. The IF has been used at 1:800 on mouse G57B6
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SHANK1
Alternate Names
SH3 and multiple ankyrin repeat domains 1, SH3 and multiple ankyrin repeat domains protein 1, Shank1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIPSPANK-1, synamon
Gene Symbol
SHANK1
Additional SHANK1 Products
Product Documents for SHANK1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SHANK1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SHANK1 Antibody - BSA Free
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Protocols
View specific protocols for SHANK1 Antibody - BSA Free (NB300-167):
SHANK1 Antibody:
Immunofluorescence
Stained Species: Mouse C57B6
Fixation: 15 minute 4% paraformaldehyde fixation
Antibody dilution: 1:800 overnight.
Secondary dilution: Alexa 568 1:500 for 45 minutes
Tissue preparation: Blocked for 2 hrs at room temp in 10% normal goat serum and Triton X-100 (0.5%) in 0.1 M Phosphate buffered saline (PBS)
Washed: 3X 0.1 M PBS between primary and secondary step. Mounted tissue with Paramount.
Confocal: Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack
Immunofluorescence
Stained Species: Mouse C57B6
Fixation: 15 minute 4% paraformaldehyde fixation
Antibody dilution: 1:800 overnight.
Secondary dilution: Alexa 568 1:500 for 45 minutes
Tissue preparation: Blocked for 2 hrs at room temp in 10% normal goat serum and Triton X-100 (0.5%) in 0.1 M Phosphate buffered saline (PBS)
Washed: 3X 0.1 M PBS between primary and secondary step. Mounted tissue with Paramount.
Confocal: Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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