SHANK1 Antibody - BSA Free

Novus Biologicals | Catalog # NB300-167

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.

Reactivity Notes

Human, rat and mouse. Other species have not been tested.

Specificity

Reacts with residues [SGPIYPGLFDIRSS] of the C-terminus of the SHANK1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHANK1 Antibody - BSA Free

Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

SHANK1-Antibody-Immunohistochemistry-NB300-167-img0012.jpg
Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.
Western Blot: SHANK1 AntibodyBSA Free [NB300-167]

Western Blot: SHANK1 AntibodyBSA Free [NB300-167]

Western Blot: SHANK1 Antibody [NB300-167] - Shank1a in rat brain lysate using NB300-167 at 1:500 dilution.
Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

SHANK1-Antibody-Immunohistochemistry-NB300-167-img0013.jpg
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167]

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167]

SHANK1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-167-img0010.jpg
Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

SHANK1-Antibody-Immunohistochemistry-NB300-167-img0014.jpg
Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167]

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167]

SHANK1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-167-img0011.jpg
Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

SHANK1-Antibody-Immunohistochemistry-NB300-167-img0017.jpg
Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody - BSA Free [NB300-167]

Immunohistochemistry: SHANK1 Antibody [NB300-167] - Retinal tissue using NB300-167 at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.
SHANK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Top box: 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar = 300 μm. Bottom box: same stainings as top box but with the VGLUT2 antibody (white; red in merge). Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 μm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SHANK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Top box: 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar = 300 μm. Bottom box: same stainings as top box but with the VGLUT2 antibody (white; red in merge). Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 μm. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SHANK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank puncta with VGLUT2 puncta in the VGLUT2-band nor in the intragranular mossy fibers of the dentate gyrus. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: dentate Gyrus = DG, containing stratum granulare = SG, stratum moleculare = SM, & polymorphic layer = PL; I. indicates uppermost row/figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge), & VGLUT2 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 μm), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the outer part of the SG (2, top row) & in the PL (1, bottom row) (scale bar = 5 μm). Red arrows indicate VGLUT2-band. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SHANK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the stratum radiatum of the CA1. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA1 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR; I./II./III. indicates uppermost/middle/bottom figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SHANK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers & the stratum moleculare of the dentate gyrus. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: dentate Gyrus = DG, containing stratum granulare = SG, stratum moleculare = SM, & polymorphic layer = PL; I. indicates uppermost row/figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 μm), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the PL (1, top row) & SM (3, bottom row) (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SHANK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank immunoreactivity with VGLUT2 puncta in the VGLUT2-band of the stratum pyramidale nor in the mossy fibers of the CA3. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA3 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT2 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows further enlargements (indicated in upper row by white rectangles) in the lateral part of the SP (2, top row) & the SR (1, bottom row) (scale bar = 5 μm). Red arrows indicate VGLUT2-band. Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SHANK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] -

Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank immunoreactivity with VGLUT1 in the stratum radiatum/stratum lucidum of the CA3 region. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA3 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (http://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SHANK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:800

Immunohistochemistry

1:250-1:750

Immunohistochemistry-Paraffin

1:250-1:750

Western Blot

1:500-1:1000
Application Notes
This antibody is useful for Western blot, immunohistochemistry-Paraffin, and immunohistochemistry. The IF has been used at 1:800 on mouse G57B6

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHANK1

Shank proteins are a family of scaffold proteins identified through their interaction with a variety of membrane and cyto-plasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are crucial in receptor tyrosine kinase signaling. Recent studies suggest that disruption of glutamate receptors at the Shank-postsynaptic platform could contribute to the destruction of post-synaptic density which underlies synaptic dysfunction and loss in Alzheimers disease. Shank1 might be relevant to human autism spectrum disorders due to its differential role in specific cognitive processes along with its importance in synapse structure and function.

Alternate Names

SH3 and multiple ankyrin repeat domains 1, SH3 and multiple ankyrin repeat domains protein 1, Shank1, Somatostatin receptor-interacting protein, SSTR-interacting protein, SSTRIPSPANK-1, synamon

Entrez Gene IDs

50944 (Human); 243961 (Mouse); 78957 (Rat)

Gene Symbol

SHANK1

Additional SHANK1 Products

Product Documents for SHANK1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SHANK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SHANK1 Antibody - BSA Free

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Protocols

View specific protocols for SHANK1 Antibody - BSA Free (NB300-167):

SHANK1 Antibody:
Immunofluorescence

Stained Species: Mouse C57B6
Fixation: 15 minute 4% paraformaldehyde fixation
Antibody dilution: 1:800 overnight.
Secondary dilution: Alexa 568 1:500 for 45 minutes
Tissue preparation: Blocked for 2 hrs at room temp in 10% normal goat serum and Triton X-100 (0.5%) in 0.1 M Phosphate buffered saline (PBS)
Washed: 3X 0.1 M PBS between primary and secondary step. Mounted tissue with Paramount.
Confocal: Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack


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