SHANK3 Antibody

Novus Biologicals | Catalog # NBP3-13162

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SHANK3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse SHANK3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for SHANK3 Antibody

Western Blot: SHANK3 Antibody [NBP3-13162]

Western Blot: SHANK3 Antibody [NBP3-13162]

Western Blot: SHANK3 Antibody [NBP3-13162] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (NBP3-13162) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody [NBP3-13162]

Immunocytochemistry/ Immunofluorescence: SHANK3 Antibody [NBP3-13162]

Immunocytochemistry/Immunofluorescence: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: SHANK3 Antibody [NBP3-13162]

Immunohistochemistry-Frozen: SHANK3 Antibody [NBP3-13162]

Immunohistochemistry-Frozen: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.

Applications for SHANK3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHANK3

The SHANK3 gene encodes a SH3 and multiple ankyrin repeat domain protein 3 that in isoform 1 is 1,741 amino acids long at 186 kDA and in isoform 2 is 1,732 amino acids long at 185 kDA. This protein functions as an adaptor in postsynaptic density of excitatory synapses. Through complexes that include GKAP/PSD-95 and Homer and the actin-based cytoskeleton, these excitatory synapses are connected with the postsynaptic membrane including NMDA-type and metabotropic glutamate receptor. Additionally, this protein may function in structural and organizational projects of the dendritic spine and the synaptic junction. SHANK3 gene participates in cytoplasmic microtubules, glutamatergic synapses, and signaling events regulated by ret tyrosine kinase. It is known to interact with genes PLCG1, FYN, SRC, ABL1, and PIK3R1. Defects in SHANK3 are associated with autism spectrum disorders and schizophrenia type 15. It is also associated with phelan-mcdermid syndrome, cerebritis, prostatitis, neuronitis, dementia, prostate cancer, hypogonadism, hepatitis a, jacobsen syndrome, and trisomy 22.

Alternate Names

DEL22q13.3, KIAA1650proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2SH3 and multiple ankyrin repeat domains protein 3, SH3 and multiple ankyrin repeat domains 3, shank postsynaptic density protein, Shank3, SPANK-2

Gene Symbol

SHANK3

Additional SHANK3 Products

Product Documents for SHANK3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SHANK3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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