SHIP Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-49633

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SHIP Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MPRKEPPPCPEPGILSPSIVLTKAQEADRGEGPGKQVPAPRLRSFTCSSSAEGRAAGGDKSQGKPKTPVSSQAPVPAKRPIKPSRSEINQQT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SHIP Antibody - BSA Free (NBP2-49633) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SHIP Antibody - BSA Free

SHIP Antibody

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] -

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] - Analysis in human tonsil and skeletal muscle tissues using NBP2-49633 antibody. Corresponding INPP5D RNA-seq data are presented for the same tissues.
Western Blot: SHIP Antibody [NBP2-49633]

Western Blot: SHIP Antibody [NBP2-49633]

Western Blot: SHIP Antibody [NBP2-49633] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 4: Human plasma
Lane 5: Human Liver tissue
Lane 6: Human Tonsil tissue
Immunocytochemistry/ Immunofluorescence: SHIP Antibody [NBP2-49633]

Immunocytochemistry/ Immunofluorescence: SHIP Antibody [NBP2-49633]

Immunocytochemistry/Immunofluorescence: SHIP Antibody [NBP2-49633] - Staining of human cell line Hep G2 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry: SHIP Antibody [NBP2-49633]

Immunohistochemistry: SHIP Antibody [NBP2-49633]

Immunohistochemistry: SHIP Antibody [NBP2-49633] - Staining of human small intestine shows strong cytoplasmic positivity in lymphoid cells.
SHIP Antibody

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] -

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] -Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.
SHIP Antibody

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] -

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] -Staining of human liver shows no positivity in hepatocytes as expected.
SHIP Antibody

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] -

Immunohistochemistry-Paraffin: SHIP Antibody [NBP2-49633] -Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for SHIP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHIP

SH2-containing inositol phosphatase 1 (SHIP) is a 145 kDa hemopoietic-specific phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3, 4-bisphosphate. SHIP has been shown to bind directly a phosphorylated motif, immunoreceptor tyrosine-based inhibition motif (ITIM), present in the cytoplasmic domain of FcgammaRIIB1 (1). Also, results demonstrate that FcgammaRIIB use SHIP1 to inhibit pathways shared by receptor tyrosine kinases and immunoreceptors to trigger cell proliferation and cell activation, respectively (2).

Long Name

SH2-containing Inositol Phosphatase

Alternate Names

INPP5D

Gene Symbol

INPP5D

Additional SHIP Products

Product Documents for SHIP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SHIP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SHIP Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SHIP Antibody - BSA Free and earn rewards!

Have you used SHIP Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies