Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SHMT1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 453 to 483 of human Serine Hydroxymethyltransferase 1 using the numbering given in entry NP_004160.3 (GeneID 6470).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHMT1 Antibody - BSA Free

Western Blot: SHMT1 Antibody [NBP2-32173]

Western Blot: SHMT1 Antibody [NBP2-32173]

Western Blot: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody NBP2-32173 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP2-32173]

Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP2-32173]

Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP2-32173] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti- SHMT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Western Blot: SHMT1 Antibody [NBP2-32173]

Western Blot: SHMT1 Antibody [NBP2-32173]

Western Blot: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 NBP2-32173 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP2-32173]

Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP2-32173]

Immunohistochemistry-Paraffin: SHMT1 Antibody [NBP2-32173] - Section of human lung cancer. Antibody: Affinity purified rabbit anti- SHMT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: SHMT1 Antibody [NBP2-32173]

Immunoprecipitation: SHMT1 Antibody [NBP2-32173]

Immunoprecipitation: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody NBP2-32173 used for IP at 6 ug per reaction. SHMT1 was also immunoprecipitated by rabbit anti-SHMT1 antibody BL15560. For blotting immunoprecipitated SHMT1, NBP2-32173 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SHMT1 Antibody - BSA Free

Application
Recommended Usage

ELISA

Reported in scientific literature (PMID 27695002).

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:500 - 1:2500
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SHMT1

SHMT1 encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.

Long Name

Serine hydroxymethyltransferase, cytosolic

Alternate Names

EC 2.1.2.1, Serine methylase, SHMT

Gene Symbol

SHMT1

UniProt

NP_004160.3 (Human)

Additional SHMT1 Products

Product Documents for SHMT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SHMT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SHMT1 Antibody - BSA Free

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Protocols

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