SHMT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20354

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%), Mouse (93%), Rabbit (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SHMT2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SHMT2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (81%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHMT2 Antibody - BSA Free

Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP2-20354]

Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP2-20354]

Immunohistochemistry-Paraffin: SHMT2 Antibody [NBP2-20354] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.
Western Blot: SHMT2 Antibody [NBP2-20354]

Western Blot: SHMT2 Antibody [NBP2-20354]

Western Blot: SHMT2 Antibody [NBP2-20354] - Sample (whole cell lysate) A: 293T 10ug, B: HeLa 10ug, 7. 5% SDS PAGE gel, diluted at 1:10000.
SHMT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SHMT2 Antibody [NBP2-20354] -

SHMT2 antibody detects SHMT2 protein at Mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 1 sec.
Green: SHMT2 protein stained by SHMT2 antibody (NBP2-20354) diluted at 1:500.
Blue: Hoechst 33342 staining.
SHMT2 Antibody - BSA Free

Western Blot: SHMT2 Antibody [NBP2-20354] -

SHMT2 antibody detects SHMT2 protein by Western blot analysis.
A. 30 ug A431 whole cell lysate/extract
B. 30 ug HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
SHMT2 antibody (NBP2-20354) dilution: 1:5000

Applications for SHMT2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SHMT2

This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Long Name

Serine hydroxymethyltransferase, mitochondrial

Alternate Names

EC 2.1.2.1, GLY A+, GLYA, glycine auxotroph A, human complement for hamster, Glycine hydroxymethyltransferase, serine aldolase, serine hydroxymethylase, serine hydroxymethyltransferase 2 (mitochondrial), serine hydroxymethyltransferase, mitochondrial, Serine methylase, SHMT, threonine aldolase

Gene Symbol

SHMT2

Additional SHMT2 Products

Product Documents for SHMT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SHMT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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