SIAH2 Antibody (24E6H3) - BSA Free

Novus Biologicals | Catalog # NB110-88113

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Drosophila, Mouse (Negative)

Cited:

Human, Mouse, Insect - Drosophila

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24E6H3

Format

BSA Free
View all SIAH2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an N-terminal portion of the drosophila SINA protein (within residues 1-100). [Swiss-Prot# P21461]

Localization

Nuclear, Cytoplasmic

Specificity

This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SIAH2 Antibody (24E6H3) - BSA Free

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in human colorectal adenocarcinoma using NB110-88113. Staining of SIAH was not observed in normal colon epithelium. Photos courtesy of Dr. Amy Tang, Mayo Clinic Cancer Center.
Immunocytochemistry/ Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunocytochemistry/ Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunocytochemistry/Immunofluorescence: SIAH2 Antibody (24E6H3) [NB110-88113] - Staining of SIAH in ovarian carcinoma (OVCR3) cells using NB110-88113.
Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

SIAH2-Antibody-24E6H3-Immunohistochemistry-NB110-88113-img0006.jpg
Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113]

Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113]

Flow (Intracellular): SIAH2 Antibody (24E6H3) [NB110-88113] - Human HepG2 cell line was stained with Mouse Anti- SIAH2 Monoclonal Antibody (Catalog# NB110-88113, orange), or Mouse IgG1 isotype control (Catalog# MAB002, blue) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog# FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog# FC005).
Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

SIAH2-Antibody-24E6H3-Immunohistochemistry-NB110-88113-img0008.jpg
Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113]

SIAH2-Antibody-24E6H3-Immunohistochemistry-NB110-88113-img0007.jpg
SIAH2 Antibody (24E6H3)

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] -

Immunohistochemistry: SIAH2 Antibody (24E6H3) [NB110-88113] - Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, & TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SIAH2 Antibody (24E6H3) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10

Immunocytochemistry/ Immunofluorescence

1:10

Immunohistochemistry

1:40-1:50

Immunohistochemistry-Frozen

1:40-1:50

Immunohistochemistry-Paraffin

1:40-1:50

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SIAH2

The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.

Alternate Names

E3 ubiquitin-protein ligase SIAH2, EC 6.3.2, EC 6.3.2.-, hSiah2, seven in absentia (Drosophila) homolog 2, Seven in absentia homolog 2, seven in absentia homolog 2 (Drosophila), Siah-2

Entrez Gene IDs

6478 (Human); 20439 (Mouse)

Gene Symbol

SIAH2

Additional SIAH2 Products

Product Documents for SIAH2 Antibody (24E6H3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SIAH2 Antibody (24E6H3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SIAH2 Antibody (24E6H3) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SIAH2 Antibody (24E6H3) - BSA Free

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  • Q: Hi, we are trying to find out if the antibody NB110-88113 (SIAH2 Antibody (24E6H3) 0.5ml Cancer Marker, Stem Cell Marker) will be useful for our application. We want to try to look at Siah (sina in Drosophila) in drosophila larval brains, more specifically in the Drosophila neural stem cells. As in the webpage it says that this antibody recognizes Drosophila Sina we would like to ask if this antibody was tested in Drosophila tissues or Drosophila Cells.

    A:

    Our SIAH2 antibody with catalogue number NB110-88113 is covered by our 100% guarantee for the detection of SIAH2 in samples from Drosophila by Western blotting, Flow Cytometry, Immunofluorescence, and Immunohistochemistry with both frozen and paraffin-embedded samples. For further information on our guarantee please see the following link: Quality Assurance. The immunogen used to generate this antibody was a synthetic peptide made to an N-terminal portion (within residues 1-100) of the drosophila SINA protein (Swiss-Prot# P21461). Although we show data from human samples on our webpage for this antibody, we unfortunately do not have any images from Drosophila samples that I can share with you at the present time.

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