Siglec-3/CD33 Antibody (2C6B7) - BSA Free

Novus Biologicals | Catalog # NBP2-37388

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C6B7

Format

BSA Free
View all Siglec-3/CD33 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Siglec-3/CD33 (AA: 15-237) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Siglec-3/CD33 Antibody (2C6B7) - BSA Free

Western Blot: Siglec-3/CD33 Antibody (2C6B7)BSA Free [NBP2-37388]

Western Blot: Siglec-3/CD33 Antibody (2C6B7)BSA Free [NBP2-37388]

Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against HEK293 (1) and CD33 (AA: 15-237)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded endometrium tissues using CD33 mouse mAb with DAB staining.
Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

Flow Cytometry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of HepG2 cells using CD33 mouse mAb (green) and negative control (purple).
Western Blot: Siglec-3/CD33 Antibody (2C6B7)BSA Free [NBP2-37388]

Western Blot: Siglec-3/CD33 Antibody (2C6B7)BSA Free [NBP2-37388]

Western Blot: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis using CD33 mAb against human CD33 recombinant protein. (Expected MW is 49.2 kDa)
Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

Immunohistochemistry: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Analysis of paraffin-embedded esophageal cancer tissues using CD33 mouse mAb with DAB staining.
ELISA: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

ELISA: Siglec-3/CD33 Antibody (2C6B7) - BSA Free [NBP2-37388]

ELISA: Siglec-3/CD33 Antibody (2C6B7) [NBP2-37388] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Siglec-3/CD33 Antibody (2C6B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-3/CD33

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody (2C6B7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Siglec-3/CD33 Antibody (2C6B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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