Siglec-3/CD33 Antibody (PSH02-51)

Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147]

Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution.

Lane 1: TF-1 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: SK-MEL-28 cell lysate (negative)

Lysates/proteins at 20 ug/Lane.

Predicted band size: 40 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147]

Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147]

Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).