Siglec-3/CD33 Antibody (WM53) - Low Endotoxin

Novus Biologicals | Catalog # NBP3-09095

Clone WM53 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # WM53

Format

Low Endotoxin
View all Siglec-3/CD33 Primary Antibodies »

Product Specifications

Immunogen

Human AML cells

Specificity

The mouse monoclonal antibody WM53 reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Siglec-3/CD33 Antibody (WM53) - Low Endotoxin (NBP3-09095) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Endotoxin Level

Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Scientific Data Images for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Mass cytometry (surface staining) of human peripheral blood with anti-CD33 (WM53) 149Sm. Gated on singlets.
Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095]

Flow Cytometry: Siglec-3/CD33 Antibody (WM53) - Low Endotoxin, Azide and BSA Free [NBP3-09095] - Analysis (surface staining) of human peripheral blood with anti-CD33 (WM53) purified antibody (low endotoxin), GAM-APC.

Applications for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

Low Endotoxin

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-3/CD33

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Siglec-3/CD33 Antibody (WM53) - Low Endotoxin

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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