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Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Name: Simple Plex Human IFN-gamma (3rd Gen) Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-002574
Price: 885 USD
Availability: InStock
Rating: 5 (1 reviews)

R&D Systems | Catalog # SPCKB-PS-002574

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Product Documents

Simple Plex Human IFN gamma 3rd gen assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum) and CS (Cell Culture Supernatant).

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.05 pg/mL

Assay Range

0.17-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human IFN-gamma (3rd Gen) Cartridge Summary

Simple Plex assay for the detection of human IFN-gamma in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) on Ella automated benchtop ELISA.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IFN-gamma Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IFN-gamma.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	39
Mean (pg/mL)	21.1	1175	23.4	1192
Standard Deviation	1.48	33.4	2.52	99.4
CV%	7.0	2.8	10.8	8.3

Recovery for Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cerebrospinal Fluid (n=6)	94	91-98
EDTA Plasma (n=4)	89	88-90
Heparin Plasma (n=4)	90	83-99
Serum (n=4)	95	83-102

Linearity

Samples containing and/or spiked with high concentrations of human IFN-gamma were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Human IFN-gamma Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.