Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Simple Plex Human IFN gamma assay kit for use on Ella automated benchtop ELISA. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-002574
SPCKA-PS-002574
SPCKC-PS-002574
ST01B-PS-002574
Control Products Available
Human IFN-gamma Simple Plex Cartridge Kit for use with the Ella Automated Immunoassay System
3 Images
Product Details
FAQs
Supplemental Products
Reviews (1)

Simple Plex Human IFN-gamma (3rd Gen) Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (5 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity
0.17 pg/mL
Assay Range
0.2 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Specificity
Natural and recombinant human IFN-gamma.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-6 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) on Ella automated benchtop ELISA. 

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 21.1 1175 23.4 1192
Standard Deviation 1.48 33.4 2.52 99.4
CV% 7 2.8 10.8 8.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cerebrospinal Fluid (n=6) 94 91-98
EDTA Plasma (n=4) 89 88-90
Heparin Plasma (n=4) 90 83-99
Serum (n=4) 95 83-102

Linearity

Samples containing and/or spiked with high concentra- tions of human IFN-gamma were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
IFN-gamma Simple Plex Linearity

Scientific Data

IFN-gamma Simple Plex Assay Standard Curve

Product Datasheets

You must select a language.

x

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:
Interferon gamma
Entrez Gene IDs:
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

FAQs

No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs

Automated ELISA Instruments

Simple Plex Assay Controls

Reviews for Simple Plex Human IFN-gamma (3rd Gen) Cartridge

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Simple Plex Human IFN-gamma (3rd Gen) Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Simple Plex Human IFN-gamma (3rd Gen) Cartridge
By Lauren Macaisa on 07/19/2021
Sample Tested: Human cells