Simple Plex Mouse IFN-gamma Cartridge

Name: Simple Plex Mouse IFN-gamma Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-001945
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-001945

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Product Documents

Simple Plex Mouse IFN-gamma assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (25 µL), Mouse EDTA Plasma (25 µL), Mouse Heparin Plasma (25 µL)

Sensitivity

0.48 pg/mL

Assay Range

0.94-9000 pg/mL (Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Simple Plex Mouse IFN-gamma Cartridge Summary

Simple Plex assay is for the detection of mouse Interferon gamma (IFN-gamma ) in serum and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse IFN-gamma.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IFN-gamma assay.

Inter-Assay Precision (Precision between assays) Replicates of each IFN-gamma control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IFN-gamma.

Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	51	51
Mean (pg/mL)	44.4	2296	51.7	2486
Standard Deviation	1.33	205	5.35	263
CV%	3.0	8.9	10.4	10.6

Recovery for Simple Plex Mouse IFN-gamma Cartridge

Recovery for the mouse IFN-gamma assays was evaluated at three different spiked concentrations of IFN-gamma protein.

Sample Type	Average % Recovery	Range %
Mouse EDTA Plasma (n=4)	118	112-123
Mouse Heparin Plasma (n=4)	116	112-122
Mouse Serum (n=4)	114	107-118

Linearity

Samples containing and/or spiked with high concentrations of mouse IFN- gamma were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Mouse IFN-gamma Cartridge

Simple Plex Mouse IFN-gamma Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IFN-gamma Assay. Assay range is 0.94-9000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.