Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge

Name: Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-003377
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-003377

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Simple Plex Mouse IL-1 beta/IL-1F2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.13 pg/mL

Assay Range

0.88-3360 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge Summary

Simple Plex™ assay is for the detection of mouse Interleukin 1 beta (IL-1 beta /IL-1F2) in serum and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-1 beta/IL-1F2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse IL-1 beta /IL-1F2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-1 beta /IL-1F2 assay.

Inter-Assay Precision (Precision between assays) eplicates of each IL-1 beta /IL-1F2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-1 beta /IL-1F2.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	135	144
Mean (pg/mL)	54.8	2711	51.1	2529
Standard Deviation	1.87	75.3	6.22	228
CV%	3.4	2.8	12.2	9.0

Recovery for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge

Recovery for the mouse IL-1 beta /IL-1F2 assays was evaluated at three different spiked concentrations of IL-1 beta /IL-1F2 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	105	88-118
Heparin Plasma (n=4)	105	100-112
Serum (n=4)	89	83-97

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge

Simple Plex Mouse IL-1 beta /IL-1F2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-1 beta /IL-1F2 Assay. Assay range is 0.88-3360 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.