Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge

Name: Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-003377
Price: 600 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-003377

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Product Documents

Simple Plex Mouse IL-1 beta/IL-1F2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.13 pg/mL

Assay Range

0.88-3360 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge Summary

Simple Plex assay for the detection of mouse IL-1 beta in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse IL-1 beta

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	135	144
Mean (pg/mL)	54.8	2711	51.1	2529
Standard Deviation	1.87	75.3	6.22	228
CV%	3.4	2.8	12.2	9.0

Recovery for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	105	88-118
Heparin Plasma (n=4)	105	100-112
Serum (n=4)	89	83-97

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-1 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Mouse IL-1b/IL-1F2 Simple Plex Assay Standard Curve

Scientific Data Images for Simple Plex Mouse IL-1 beta/IL-1F2 Cartridge

Mouse IL-1 beta /IL-1F2 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.