SIN3A Antibody (8V5B6)

Novus Biologicals | Catalog # NBP3-16124

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8V5B6 expressed in HEK293
View all SIN3A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1174-1273 of human SIN3A (Q96ST3). YKMVYVIKSEDYMYRRTALLRAHQSHERVSKRLHQRFQAWVDKWTKEHVPREMAAETSKWLMGEGLEGLVPCTTTCDTETLHFVSINKYRVKYGTVFKAP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SIN3A Antibody (8V5B6)

Western Blot: SIN3A Antibody (8V5B6) [NBP3-16124]

Western Blot: SIN3A Antibody (8V5B6) [NBP3-16124]

Western Blot: SIN3A Antibody (8V5B6) [NBP3-16124] - Western blot analysis of extracts of various cell lines, using SIN3A antibody (NBP3-16124) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
SIN3A Antibody (8V5B6)

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124] - Confocal imaging of NIH/3T3 cells using SIN3A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded human small intestine tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded rat testis tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse brain tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded rat colon tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded rat spleen tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse colon tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded mouse testis tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
SIN3A Antibody (8V5B6)

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunocytochemistry/ Immunofluorescence: SIN3A Antibody (8V5B6) [NBP3-16124] - Confocal imaging of U-2 OS cells using SIN3A Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
SIN3A Antibody (8V5B6)

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] -

Immunohistochemistry: SIN3A Antibody (8V5B6) [NBP3-16124] - Immunohistochemistry analysis of SIN3A in paraffin-embedded human esophagus tissue using SIN3A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for SIN3A Antibody (8V5B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIN3A

Sin3A is encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

Long Name

SIN3 Homolog A

Alternate Names

DKFZp434K2235, FLJ90319, Histone deacetylase complex subunit Sin3a, KIAA0700, paired amphipathic helix protein Sin3a, SIN3 homolog A, transcription regulator (yeast), SIN3 homolog A, transcriptional regulator (yeast), Transcriptional corepressor Sin3a, transcriptional co-repressor Sin3A, transcriptional regulator, SIN3A

Gene Symbol

SIN3A

Additional SIN3A Products

Product Documents for SIN3A Antibody (8V5B6)

Certificate of Analysis

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Product Specific Notices for SIN3A Antibody (8V5B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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