Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80949
Key Product Details
Species Reactivity
Human, Mouse, Rat, Primate, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, ELISA, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1F3
Format
Azide and BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free
![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3)Azide and BSA Free [NBP2-80949]](https://resources.rndsystems.com/images/products/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Western-Blot-NBP2-80949-img0001.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody (1F3)Azide and BSA Free [NBP2-80949]")
Western Blot: Sirtuin 1/SIRT1 Antibody (1F3)Azide and BSA Free [NBP2-80949]
Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]](https://resources.rndsystems.com/images/products/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80949-img0003.jpg "Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]")
Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]
Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]](https://resources.rndsystems.com/images/products/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80949-img0004.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]")
Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]
Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining. Image from the standard format of this antibody.![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]](https://resources.rndsystems.com/images/products/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80949-img0006.jpg "Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]")
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]](https://resources.rndsystems.com/images/products/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80949-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]")
Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]
Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]](https://resources.rndsystems.com/images/products/Sirtuin-1-SIRT1-Antibody-1F3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80949-img0005.jpg "Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]")
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.Applications for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:10-1:1000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry Free-Floating
reported in scientific literature (PMID 26327687)
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Sirtuin 1/SIRT1
Long Name
Silent Mating Type Information Regulation 2 Homolog
Alternate Names
SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1
Gene Symbol
SIRT1
Additional Sirtuin 1/SIRT1 Products
Product Documents for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Notch Signaling Pathways
