Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free

Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641]

Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641]

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641]

Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.

Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641]

Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641]

Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] -

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain & blood & reduces the SIRT1 protein level in different brain regions. (a) The mRNA expression levels (relative expression levels=fold changes over control, normalized to PGK1 & TBP (Tata Binding Protein) genes) of SIRT1 & SIRT6 were significantly decreased in the brain of ESI compared with control mice. (b) In peripheral blood mononuclear cells (PBMCs) of ESI-treated mice, the expression level of SIRT1 mRNA was downregulated. ESI, n=9 (male (M)=6, female (F)=3); Control, n=7 (M=4, F=3) ***P<0.001; *P<0.05. (c) Confocal images showing SIRT1 (red) & NeuN (green) immunostaining plus 4,6-diamidino-2-phenylindole (DAPI) counterstaining (gray) in the brain of control mice. SIRT1 (red) colocalizes both with the neuronal marker NeuN (green) & with DAPI (gray). (d) Confocal images from the hippocampus & basolateral amygdala of control & ESI mice reacted with SIRT1 antibody. (e) Densitometric analysis of SIRT1 immunoreactivity in the motor cortex, striatum, hippocampus & basolateral amygdala revealed a significant reduction of SIRT1 immunoreactivity in ESI compared with control mice. The F/A ratio defines the mean fluorescence of individual samples (F) normalized to total surface (A). ESI, n=5; Control, n=5. ***P<0.001. Scale bars: (d) 100 μm; 25 μm. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/tp2015125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] -

Effects of Doxo stimulation on oxidative stress markers in hAC. (A) Gene expression of iNOS, NOX2, and NOX4 as well as CAT, SIRT1, SOD1, and SOD2 after Doxo stimulation for 10 d. (B) Exemplary western blot analysis of SIRT1 and SOD2 after Doxo stimulation (n = 2); a-TUB = alpha tubulin. NO release into culture media of Doxo-treated hAC after 5 d and 10 d, respectively. (D) Representative images of DCFDA staining of unstimulated and Doxo-stimulated hAC after 7 d and corresponding quantification of the corrected total cell fluorescence (CTFC). (E) Exemplary co-staining of mitochondrial superoxide (MitoSOX; red) and cytoplasmic ROS (DCFDA; green) of unstimulated (Ctrlol) and Doxo-stimulated hAC after 7 d. Ctrl = unstimulated hAC. Data are presented as scatter plot with bars, mean with standard deviation; or box plots with median, whiskers min to max. Significant differences between groups are depicted as: **** p ≤ 0.0001; ** p ≤ 0.01. Statistical analysis: (A) One-way ANOVA, Sidak’s multiple comparison; (C) multiple t-test; (D) paired t-test. Ctrl = control (unstimulated cells), rel. = relative. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35406671), licensed under a CC-BY license. Not internally tested by Novus Biologicals.