Sirtuin 2/SIRT2 Antibody (4B11) - Azide and BSA Free

Novus Biologicals | Catalog # H00022933-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B11

Format

Azide and BSA Free
View all Sirtuin 2/SIRT2 Primary Antibodies »

Product Specifications

Immunogen

SIRT2 (AAH03012, 128 a.a. ~ 227 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSL

Reactivity Notes

Other species not tested.

Localization

Cytoplasmic for short periods of time until complexes are formed (Sir2, Sir3 and Sir4). The complexes are recruited to their chromosome targets (nuclear) via interactions with DNA binding proteins, followed by deacetylation of histones H3 and H4. Therefor

Marker

Early Oligodendrocyte Marker

Specificity

SIRT2 - sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Sirtuin 2/SIRT2 Antibody (4B11) - Azide and BSA Free

Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01]

Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01]

Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of SIRT2 expression in transfected 293T cell line by SIRT2 monoclonal antibody (M01), clone 4B11.Lane 1: SIRT2 transfected lysate(39.5 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01]

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01]

Immunocytochemistry/Immunofluorescence: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of monoclonal antibody to SIRT2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01]

Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01]

Western Blot: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Analysis of SIRT2 over-expressed 293 cell line, cotransfected with SIRT2 Validated Chimera RNAi ( Cat # H00022933-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIRT2 monoclonal antibody (M01), clone 4B11 (Cat # H00022933-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: Sirtuin 2/SIRT2 Antibody (4B11) [H00022933-M01] - Detection limit for recombinant GST tagged SIRT2 is approximately 0.1ng/ml as a capture antibody.

Applications for Sirtuin 2/SIRT2 Antibody (4B11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA and immunofluorescence.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Sirtuin 2/SIRT2

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Entrez Gene IDs

22933 (Human)

Gene Symbol

SIRT2

OMIM

604480 (Human)

UniProt

AAH03012 (Human)

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody (4B11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Sirtuin 2/SIRT2 Antibody (4B11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Epigenetics
Redox Enzymes

Citations for Sirtuin 2/SIRT2 Antibody (4B11) - Azide and BSA Free

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Protocols

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FAQs

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