SIX1 Antibody (CL0185) - BSA Free
Novus Biologicals | Catalog # NBP2-52873
Key Product Details
Validated by
Knockout/Knockdown, Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Cited:
Immunohistochemistry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0185
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from Q15475, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SIX1 Antibody (CL0185) - BSA Free
![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0010.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]")
![Western Blot: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Western-Blot-NBP2-52873-img0005.jpg "Western Blot: SIX1 Antibody (CL0185) [NBP2-52873]")
![Immunocytochemistry/ Immunofluorescence: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Immunocytochemistry-Immunofluorescence-NBP2-52873-img0001.jpg "Immunocytochemistry/ Immunofluorescence: SIX1 Antibody (CL0185) [NBP2-52873]")
Immunocytochemistry/ Immunofluorescence: SIX1 Antibody (CL0185) [NBP2-52873]
Immunocytochemistry/Immunofluorescence: SIX1 Antibody (CL0185) [NBP2-52873] - Staining in RH30 cell line with Anti-SIX1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0002.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]")
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human liver shows absence of immunoreactivity.![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0003.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]")
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human striated muscle shows strong nuclear immunoreactivity in the myocytes.![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0006.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]")
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human endocervix shows strong nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0007.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]")
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human salivary gland shows strong nuclear positivity in glandular cells.![Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]](https://resources.rndsystems.com/images/products/SIX1-Antibody-CL0185-Immunohistochemistry-Paraffin-NBP2-52873-img0008.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]")
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873]
Immunohistochemistry-Paraffin: SIX1 Antibody (CL0185) [NBP2-52873] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.Applications for SIX1 Antibody (CL0185) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SIX1
Alternate Names
BOS3, deafness, autosomal dominant 23, DFNA23, homeobox protein SIX1, sine oculis homeobox (Drosophila) homolog 1, Sine oculis homeobox homolog 1, sine oculis homeobox homolog 1 (Drosophila), SIX homeobox 1, TIP39
Gene Symbol
SIX1
Additional SIX1 Products
Product Documents for SIX1 Antibody (CL0185) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SIX1 Antibody (CL0185) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SIX1 Antibody (CL0185) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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