SLC22A1 Antibody (2C5)

Novus Biologicals | Catalog # NBP1-51684

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Electron Microscopy

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C5
View all SLC22A1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human SLC22A1 expressed in E. Coli.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28942964).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLC22A1 Antibody (2C5)

Western Blot: SLC22A1 Antibody (2C5)BSA Free [NBP1-51684]

Western Blot: SLC22A1 Antibody (2C5)BSA Free [NBP1-51684]

Western Blot: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis using SLC22A1 mAb against HEK293 (1) and SLC22A1(AA: 284-347)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: SLC22A1 Antibody (2C5) - BSA Free [NBP1-51684]

Flow Cytometry: SLC22A1 Antibody (2C5) - BSA Free [NBP1-51684]

Flow Cytometry: SLC22A1 Antibody (2C5) [NBP1-51684] - Analysis of Jurkat cells using SLC22A1 mouse mAb (green) and negative control (purple).

Applications for SLC22A1 Antibody (2C5)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
Use in IHC reported in scientific literature (PMID: 28942964). Use in Electron Microscopy reported in scientific literature (PMID: 28362799). Use in ICC/IF reported in scientific literature (PMID: 26157489).

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SLC22A1

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. (provided by RefSeq)

Long Name

Solute carrier family 22 member 1

Alternate Names

OCT1

Entrez Gene IDs

6580 (Human)

Gene Symbol

SLC22A1

UniProt

O15245 (Human)

Additional SLC22A1 Products

Product Documents for SLC22A1 Antibody (2C5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SLC22A1 Antibody (2C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

Citations for SLC22A1 Antibody (2C5)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SLC22A1 Antibody (2C5)

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  • Q: We currently have the SLC22A1 antibody (NBP1-51684), and are in the process of developing a quantitation assay with it. However, we are in need of some kind of standard for the OCT1 protein to generate a standard curve for a colorimetric ELISA. We noticed on the Novus website that the product sheet of another SLC22A1 antibody (NBP1-59464) shows the sequence of the peptide-epitope used to generate that antibody, however we cannot find any sequence data for the immunogen used to generate the antibody that we currently have. The only information we have about this immunogen is that it is a purified recombinant fragment of hSLC22A1 expressed in E. coli. So I guess our question is, do you have any information regarding this immunogen (sequence, size, etc) that you could share with us, or can we order some of the immunogen so that we may use this as a standard for quantitating expressed OCT1? Any information would be greatly appreciated!

    A: The immunogen used to create this antibody lies between amino acids 284-347. Unfortunately this immunogen is not available for purchase, however.

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