SLC34A1 Antibody (10B1.3E9) - BSA Free

Novus Biologicals | Catalog # NBP2-42216

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 10B1.3E9

Format

BSA Free
View all SLC34A1 Primary Antibodies »

Product Specifications

Immunogen

Partial synthetic peptide made to an internal portion of the human SLC34A1 protein (between amino acids 25-150) [UniProt Q06495]

Localization

Membrane-Cytoplasmic

Marker

Tubulointerstitium Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

68.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SLC34A1 Antibody (10B1.3E9) - BSA Free

Western Blot: SLC34A1 Antibody (10B1.3E9)BSA Free [NBP2-42216]

Western Blot: SLC34A1 Antibody (10B1.3E9)BSA Free [NBP2-42216]

Western Blot: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - WB detection of SLC34A1 /NPTIIa protein in a lysate of mouse thymus using SLC34A1 clone 10B1.3E9 at its 1:500 dilution. The antibody detected a single specific band at ~80 kDa representing the glycosylated form of SLC34A1 protein.
Immunocytochemistry/ Immunofluorescence: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Immunocytochemistry/ Immunofluorescence: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Immunocytochemistry/Immunofluorescence: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized using 1X TBS + 0.5% Triton-X100. The cells were incubated with hSLC34A1 (10B1.3E9) at a 1:50 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green). Actin was detected with Phalloidin 568 (Red). Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Immunohistochemistry-Paraffin: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Immunohistochemistry-Paraffin: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - IHC analysis of a formalin-fixed and paraffin-embedded tissue section of human normal kidney using SLC34A1 antibody (clone 10B1.3E9) at 1:75 dilution. The epithelial cells of various renal ducts and tubules depicted very nice membrane-cytoplasmic SLC34A1 immunostaining while the Bowman's capsule and the nuclei of cells were largely negative for SLC34A1 protein.
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - FLOW detection of SLC34A1 protein on HEK293 cells - After fixation and permeabilization, 2 x 10^6 cells/ml were stained using SLC34A1 antibody (clone 10B1.3E9) at 1:1000 dilution. Signal was developped using GtxMs dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur.
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) - BSA Free [NBP2-42216]

Flow Cytometry: SLC34A1 Antibody (10B1.3E9) [NBP2-42216] - An intracellular stain was performed on Hek293 cells with SLC34A1 Antibody [10B1.3E9] NBP2-42216PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Applications for SLC34A1 Antibody (10B1.3E9) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Immunoprecipitation

reported in scientific literature (PMID 35307350)

Western Blot

1:500
Application Notes
SLC34A1 is a 639 amino acids long protein with predicted molecular weight of 68.9 kDa, however, because of glycosylation, the processed protein may show up at higher than predicted molecular weight position on Western blot.

Reviewed Applications

Read 1 review rated 5 using NBP2-42216 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SLC34A1

SLC34A1 / NaPi-IIa [Solute carrier family 34 (type II sodium/phosphate cotransporter), member 1] belongs to the type II sodium-phosphate cotransporter family and is a multi-pass membrane protein critically involved in the maintenance of renal inorganic phosphate (Pi) homeostasis and the plasma Pi levels. SLC34A1 acts as the most significant regulator of Pi homeostasis through its reabsorption via Na(+) cotransport in the brush border membrane of renal tissues. It is mainly expressed in the kidney wherein it accounts for 70-80% of the apical influx, however, its presence in some other tissues such as lungs, testes, fallopian tubes, and heart myocytes has also been documented (UniProt and Human Protein Atlas project databases). Signaling implicated in SLC34A1's regulation include the protein kinases A /C, ERK1/2, Klotho/KL, the PI3K/PKB/GSK3 kinase cascade, NHERF1/ SLC9A3R1, AMP-activated protein kinase AMPK, JAK2, and B-RAF. Moreover, SLC34A1 expression has been reported to be controlled by dietary Pi and hormones namely 1,25-dihydroxyvitamin D3, FGF23, Dopamine, Thyroid Hormone, Glucocorticoids, Estrogen /E2 and Triiodothyronine/T3. Besides renal Pi homeostasis, SLC34A1 has been suggested to be involved in several biological processes such as kidney development, bone remodeling, dentinogenesis, cholesterol metabolism, cellular response to metal ion/drug, parathyroid hormone stimulus, protein homooligomerization etc. SLC34A1 mutations have been shown to be associated with diseases such as Nephrolithiasis/osteoporosis, hypophosphatemic, 1 (NPHLOP1), Fanconi renotubular syndrome 2 (FRTS2) and idiopathic infantile hypercalcemia (IIH).

Alternate Names

FRTS2, Na(+)/Pi cotransporter 2A, Na(+)-dependent phosphate cotransporter 2A, naPi-2a, NaPi-3, NPT2NPHLOP1, NPTIIa, renal sodium-dependent phosphate transporter, SLC11, SLC17A2Na+-phosphate cotransporter type II, sodium/phosphate co-transporter, Sodium/phosphate cotransporter 2A, sodium-dependent phosphate transport protein 2A, Sodium-phosphate transport protein 2A, solute carrier family 17 (sodium phosphate), member 2, solute carrier family 34 (sodium phosphate), member 1, Solute carrier family 34 member 1

Entrez Gene IDs

6569 (Human)

Gene Symbol

SLC34A1

UniProt

Q06495 (Human)

Additional SLC34A1 Products

Product Documents for SLC34A1 Antibody (10B1.3E9) - BSA Free

Certificate of Analysis

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Product Specific Notices for SLC34A1 Antibody (10B1.3E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SLC34A1 Antibody (10B1.3E9) - BSA Free

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  • SLC34A1 Antibody (10B1.3E9)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 08/17/2021
    SLC34A1 Antibody in kidney
    SLC34A1 Antibody (10B1.3E9) - BSA Free NBP2-42216

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Protocols

View specific protocols for SLC34A1 Antibody (10B1.3E9) - BSA Free (NBP2-42216):


Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Reagents needed:
a. Washing Buffer: Tris Buffer Saline with 0.01% of tween 20).
b. Blocking Buffer: 5% skimmed milk powder in washing buffer).
c. Secondary antibody, Horseradish peroxidase conjugated.
d. Chemiluminescent solution (SuperSignal WestPicoTM, Pierce).

Western blot Method:
1. Perform SDS-PAGE using PVDF membrane. Cut into strips.
2. Activate strips with methanol by dipping them into methanol for 5 min.
3. Discard the methanol and take fresh methanol to repeat step b.
4. Let the strips dry, and then add blocking solution and incubate at RT in a shaker for 30-45 minutes.
5. Dilute primary antibody in blocking buffer. Incubate the number of strips required with the diluted primary antibody at room temperature for 2 hours in a shaker.
6. Wash strips two times with washing buffer at 30 minutes intervals.
7. Dilute HRP conjugated secondary antibody in blocking buffer. Add diluted secondary antibody to the membrane strips and incubate for exactly 1 hour while shaking at RT.
8. Wash the strips with washing buffer for 2-3 hours with 3 to 4 changes on a shaker. This helps in reducing the back ground staining.
9. Prepare the chemiluminescent solution (SuperSignal WestPicoTM) by mixing solution A and Solution B at 1:1. Mix well. Soak the strip in the chemiluminescent solution; keep for 3-5 minutes under constant shaking.
10. Expose the membrane to a sheet of film and develop.

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