SLC39A1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-76498
![Western Blot: SLC39A1 AntibodyBSA Free [NBP1-76498]](https://resources.rndsystems.com/images/products/SLC39A1-Antibody-Western-Blot-NBP1-76498-img0001.jpg "Western Blot: SLC39A1 AntibodyBSA Free [NBP1-76498]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human ZIP1. The immunogen is located within amino acids 40 - 90 of ZIP1. Amino Acid Squence: LRRPGANHEGSASRQK
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (88%)
Specificity
At least two isoforms of ZIP1 are known to exist; this antibody will detect both isoforms. ZIP1 antibody is predicted not to cross-react with other ZIP family proteins.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SLC39A1 Antibody - BSA Free
Western Blot: SLC39A1 AntibodyBSA Free [NBP1-76498]
Western Blot: SLC39A1 Antibody [NBP1-76498] - Mouse kidney tissue lysate with ZIP1 antibody at (A) 1 and (B) 2 ug/mL.![Immunohistochemistry: SLC39A1 Antibody - BSA Free [NBP1-76498]](https://resources.rndsystems.com/images/products/SLC39A1-Antibody-Immunohistochemistry-NBP1-76498-img0003.jpg "Immunohistochemistry: SLC39A1 Antibody - BSA Free [NBP1-76498]")
Immunohistochemistry: SLC39A1 Antibody - BSA Free [NBP1-76498]
Immunohistochemistry: SLC39A1 Antibody [NBP1-76498] - Human kidney tissue at 2.5 ug/mL. dilution
Immunocytochemistry/ Immunofluorescence: SLC39A1 Antibody - BSA Free [NBP1-76498] -
Immunocytochemistry/ Immunofluorescence: SLC39A1 Antibody - BSA Free [NBP1-76498] - Immunofluorescence of SLC39A1 in human kidney tissue with SLC39A1 antibody at 20 u/mL.Applications for SLC39A1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/mL
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/mL
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC39A1
Alternate Names
CGI-08, CGI-71, IRT1, solute carrier family 39 (zinc transporter), member 1, solute carrier family 39 (zinc transporter), member 3, ZIP1, ZIP-1
Gene Symbol
SLC39A1
UniProt
Additional SLC39A1 Products
Product Documents for SLC39A1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SLC39A1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SLC39A1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for SLC39A1 Antibody - BSA Free
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Q: I would like to measure Slc39a1 expression profile in my samples, rat brain tissue lysates. Do you think I can use this antibody?
A:
The SLC39A1 antibody with catalog number NBP1-76498 is covered by the Novus Guarantee to detect SLC39A1 by Western blotting, ELISA and IHC in human and mouse samples. Unfortunately we do not yet have any testing data in rat samples. The immunogen used to generate this antibody was a 16 amino acid peptide near the amino terminus of human ZIP1. However I was unable to run an alignment between the human and rat proteins for you, since the rat sequence is not yet available on UniProt. If you can provide the rat sequence I will be happy to carry out the alignment for you to check the sequence homology between human and rat. Alternatively you may be interested in our Innovators Reward Program. This allows you to try our primary antibodies in an untested species or application, without the financial risk of failure. You simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. You can also submit reviews by emailing innovators@novusbio.com.
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