SLC5A5/Sodium Iodide Symporter Antibody (FP5) - BSA Free
Novus Biologicals | Catalog # NBP1-70342
![Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]](https://resources.rndsystems.com/images/products/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Western-Blot-NBP1-70342-img0007.jpg "Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Microarray
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # FP5
Format
BSA Free
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Product Specifications
Immunogen
Mannose binding protein hNIS fusion (AA468-643)
Localization
Membrane
Specificity
Detects approx 97kDa, non-glycosylated version at 68kDa. Other minor bands associated with hNIS at 160kDa, and degradation products at approx 30 kDa, and approx 15kDa.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for SLC5A5/Sodium Iodide Symporter Antibody (FP5) - BSA Free
Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000.![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]](https://resources.rndsystems.com/images/products/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0010.jpg "Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]")
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.![Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]](https://resources.rndsystems.com/images/products/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Western-Blot-NBP1-70342-img0006.jpg "Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]")
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]](https://resources.rndsystems.com/images/products/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0008.jpg "Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]")
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]](https://resources.rndsystems.com/images/products/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0009.jpg "Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]")
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]](https://resources.rndsystems.com/images/products/SLC5A5-Sodium-Iodide-Symporter-Antibody-FP5-Immunohistochemistry-NBP1-70342-img0011.jpg "Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]")
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342]
Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (FP5) [NBP1-70342] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP1-70342). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP1-70342) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.Applications for SLC5A5/Sodium Iodide Symporter Antibody (FP5) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:1000
Western Blot
1:1000
Application Notes
1 ug/ml of Sodium-Iodide Symporter Antibody was sufficient for detection of hNIS in 20 ug of transfected COS-7 cell membrane lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SLC5A5
Long Name
Solute Carrier Family 5 (Sodium Iodide Symporter), Member 5
Alternate Names
NIS, TDH1
Entrez Gene IDs
65199 (Rat)
Gene Symbol
SLC5A5
UniProt
Additional SLC5A5 Products
Product Documents for SLC5A5/Sodium Iodide Symporter Antibody (FP5) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SLC5A5/Sodium Iodide Symporter Antibody (FP5) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for SLC5A5/Sodium Iodide Symporter Antibody (FP5) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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