Slug Antibody (4B6D5) - BSA Free

Novus Biologicals | Catalog # NBP2-52570

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4B6D5

Format

BSA Free
View all Slug Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Slug (AA: 100-200) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Slug Antibody (4B6D5) - BSA Free

Western Blot: Slug Antibody (4B6D5)BSA Free [NBP2-52570]

Western Blot: Slug Antibody (4B6D5)BSA Free [NBP2-52570]

Western Blot: Slug Antibody (4B6D5) [NBP2-52570] - Analysis using Slug mouse mAb against MCF-7 cell lysate.
Immunocytochemistry/ Immunofluorescence: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Immunocytochemistry/ Immunofluorescence: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Immunocytochemistry/Immunofluorescence: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of Hela cells using SNAI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of colon cancer tissues using SNAI2 mouse mAb with DAB staining.
Flow Cytometry: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Flow Cytometry: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Flow Cytometry: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of MCF-7 cells using SNAI2 mouse mAb (green) and negative control (red).
Western Blot: Slug Antibody (4B6D5)BSA Free [NBP2-52570]

Western Blot: Slug Antibody (4B6D5)BSA Free [NBP2-52570]

Western Blot: Slug Antibody (4B6D5) [NBP2-52570] - Analysis using SNAI2 mAb against HEK293 (1) and SNAI2 (AA: 100-200)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Slug Antibody (4B6D5)BSA Free [NBP2-52570]

Western Blot: Slug Antibody (4B6D5)BSA Free [NBP2-52570]

Western Blot: Slug Antibody (4B6D5) [NBP2-52570] - Analysis using SNAI2 mAb against human SNAI2 (AA: 100-200) recombinant protein. (Expected MW is 39.8 kDa)
Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of cervical cancer tissues using SNAI2 mouse mAb with DAB staining.
ELISA: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

ELISA: Slug Antibody (4B6D5) - BSA Free [NBP2-52570]

ELISA: Slug Antibody (4B6D5) [NBP2-52570] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for Slug Antibody (4B6D5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Slug

SLUG encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Alternate Names

SLUGH1, SNAI2, SNAIL2, WS2D

Gene Symbol

SNAI2

Additional Slug Products

Product Documents for Slug Antibody (4B6D5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Slug Antibody (4B6D5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Slug Antibody (4B6D5) - BSA Free

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Protocols

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FAQs

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