Smad2 [p Ser465, p Ser467] Antibody

Novus Biologicals | Catalog # NBP3-13273

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Porcine (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Smad2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser465/Ser467 of human Smad2. The exact sequence is proprietary.

Modification

p Ser465, p Ser467

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Smad2 [p Ser465, p Ser467] Antibody

Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273]

Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273]

Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Untreated (-) and treated (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273]

Immunocytochemistry/ Immunofluorescence: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273]

Immunocytochemistry/Immunofluorescence: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Smad2 (phospho Ser465/Ser467) antibody detects Smad2 (phospho Ser465/Ser467) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Smad2 (phospho Ser465/Ser467) stained by Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.
Smad2 [p Ser465, p Ser467] Antibody

Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Smad2 [p Ser465, p Ser467] Antibody

Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] -

Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Smad2 [p Ser465, p Ser467] Antibody

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1500

Western Blot

1:500-1:3000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad2

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents for Smad2 [p Ser465, p Ser467] Antibody

Certificate of Analysis

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Product Specific Notices for Smad2 [p Ser465, p Ser467] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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