Smad3 Antibody (7F3) - Azide and BSA Free

Novus Biologicals | Catalog # H00004088-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7F3

Format

Azide and BSA Free
View all Smad3 Primary Antibodies »

Product Specifications

Immunogen

SMAD3 (NP_005893, 120 a.a. ~ 221 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDL

Specificity

SMAD3 - SMAD, mothers against DPP homolog 3 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Smad3 Antibody (7F3) - Azide and BSA Free

Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M02), clone 7F3.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Smad3 Antibody (7F3) [H00004088-M02]

Immunohistochemistry-Paraffin: Smad3 Antibody (7F3) [H00004088-M02]

Immunohistochemistry-Paraffin: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in HeLa.
Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in PC-12.
Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in 293.
Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in Jurkat.
Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02]

Western Blot: Smad3 Antibody (7F3) [H00004088-M02] - SMAD3 monoclonal antibody (M02), clone 7F3. Analysis of SMAD3 expression in human colon.
Proximity Ligation Assay: Smad3 Antibody (7F3) [H00004088-M02]

Proximity Ligation Assay: Smad3 Antibody (7F3) [H00004088-M02]

Proximity Ligation Assay: Smad3 Antibody (7F3) [H00004088-M02] - Analysis of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with anti-MAPK8 rabbit purified polyclonal 1:1200 and anti-SMAD3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
ELISA: Smad3 Antibody (7F3) [H00004088-M02]

ELISA: Smad3 Antibody (7F3) [H00004088-M02]

ELISA: Smad3 Antibody (7F3) [H00004088-M02] - Detection limit for recombinant GST tagged SMAD3 is approximately 0.1ng/ml as a capture antibody.

Applications for Smad3 Antibody (7F3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Smad3

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Long Name

Mothers Against DPP Homolog 3

Alternate Names

DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3

Entrez Gene IDs

4088 (Human)

Gene Symbol

SMAD3

OMIM

603109 (Human)

UniProt

NP_005893 (Human)

Additional Smad3 Products

Product Documents for Smad3 Antibody (7F3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Smad3 Antibody (7F3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Smad3 Antibody (7F3) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Smad3 Antibody (7F3) - Azide and BSA Free

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  • Q: I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?

    A: We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

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