Smad4 Antibody (4G1C6)

Name: Smad4 Antibody (4G1C6)
Brand: Novus Biologicals
SKU: NBP1-51646
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-51646

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4G1C6

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Product Specifications

Immunogen

Purified recombinant fragment of human Smad4 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Smad4 Antibody (4G1C6)

Western Blot: Smad4 Antibody (4G1C6)BSA Free [NBP1-51646]

Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.

Immunocytochemistry/ Immunofluorescence: Smad4 Antibody (4G1C6) - BSA Free [NBP1-51646]

Immunocytochemistry/Immunofluorescence: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: Smad4 Antibody (4G1C6) - BSA Free [NBP1-51646]

Immunohistochemistry-Paraffin: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of lung cancer tissues using SMAD4 mouse mAb with DAB staining.

Flow Cytometry: Smad4 Antibody (4G1C6) - BSA Free [NBP1-51646]

Flow Cytometry: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).

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Applications for Smad4 Antibody (4G1C6)

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad4

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Entrez Gene IDs

4089 (Human)

Gene Symbol

SMAD4

UniProt

Q13485

Additional Smad4 Products

Product Documents for Smad4 Antibody (4G1C6)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Smad4 Antibody (4G1C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Mesenchymal to Epithelial Transition

Primordial and Mature Germ Cells

Transcription Factors in the Akt Pathway

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