SMAP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83702

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMAP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KAAEPTVDLLGLDGPAVAPVTNGNTTVPPLNDDLDIFGPMISNPLPATVMPPAQGTPSAPAAATLSTVTSGDLDLFTEQTTKSEEVAKKQLSKDSIL

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23752192)

Localization

Cytoplasm. Nucleus. Predominant in the perinuclear region. Little Protein in the nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMAP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SMAP1 Antibody [NBP1-83702]

Immunocytochemistry/ Immunofluorescence: SMAP1 Antibody [NBP1-83702]

Immunocytochemistry/Immunofluorescence: SMAP1 Antibody [NBP1-83702] - Staining of human cell line A-431 shows positivity in plasma membrane and cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMAP1 Antibody [NBP1-83702]

Immunohistochemistry-Paraffin: SMAP1 Antibody [NBP1-83702]

Immunohistochemistry-Paraffin: SMAP1 Antibody [NBP1-83702] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
SMAP1 Antibody - BSA Free Western Blot: SMAP1 Antibody - BSA Free [NBP1-83702]

Western Blot: SMAP1 Antibody - BSA Free [NBP1-83702]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
SMAP1 Antibody - BSA Free Western Blot: SMAP1 Antibody - BSA Free [NBP1-83702]

Western Blot: SMAP1 Antibody - BSA Free [NBP1-83702]

Analysis in Rh30 cells) transfected with control siRNA, target specific siRNA probe #1, using Anti-SMAP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Applications for SMAP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAP1

SMAP1 is encoded by this gene is similar to the mouse stromal membrane-associated protein-1. This similarity suggests that this human gene product is also a type II membrane glycoprotein involved in the erythropoietic stimulatory activity of stromal cells. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

FLJ13159, FLJ42245, small ArfGAP 1, SMAP-1, stromal membrane-associated GTPase-activating protein 1, stromal membrane-associated protein 1

Entrez Gene IDs

60682 (Human)

Gene Symbol

SMAP1

UniProt

Q9H3U1 (Human)

Additional SMAP1 Products

Product Documents for SMAP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMAP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMAP1 Antibody - BSA Free

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Protocols

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FAQs

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